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<PAGE> 1 EXHIBIT 23.1 INDEPENDENT AUDITORS' CONSENT The Board of Directors Third Wave Technologies, Inc. We consent to the references to our firm under the captions "Selected Financial Data" and "Experts" and to the use of our report dated January 21, 2000 in the Registration Statement (Form S-1) and related Prospectus of Third Wave Technologies, Inc. dated July 31, 2000, as amended. /s/ Ernst & Young LLP Milwaukee, Wisconsin September 8, 2000
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