JANUS INVESTMENT FUND
24F-2NT, 1995-11-17
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                     U.S. SECURITIES AND EXCHANGE COMMISSION
                             Washington, D.C. 20549

                                   Form 24f-2
                        Annual Notice of Securities Sold
                             Pursuant to Rule 24f-2


1.       Name and address of issuer:

         Janus Investment Fund
         100 Fillmore Street
         Denver, CO 80206-4923

2.       Name of each series or class of funds for which this notice is filed:

          Janus Government Money Market Fund (Investor Shares and Institutional
               Shares)
          Janus Money Market Fund (Investor Shares and Institutional Shares)
          Janus Tax-Exempt Money Market Fund (Investor Shares and Institutional 
               Shares)
          Janus Balanced Fund
          Janus Enterprise Fund
          Janus Federal Tax-Exempt Fund
          Janus Flexible Income Fund
          Janus Fund
          Janus Growth and Income Fund
          Janus Intermediate Government Securities Fund
          Janus Mercury Fund
          Janus Overseas Fund
          Janus Short-Term Bond Fund
          Janus Twenty Fund
          Janus Venture Fund
          Janus Worldwide Fund

3.       Investment Company Act File Number:  811-1879

         Securities Act File Number:  2-34393

4.       Last day of fiscal year for which this notice is filed:

         October 31, 1995


<PAGE>

5.       Check box if this  notice is being  filed  more than 180 days after the
         close of the issuer's fiscal year for purposes of reporting  securities
         sold after the close of the fiscal year but before  termination  of the
         issuer's 24f-2 declaration:

                                                                             [ ]


6.       Date of termination of issuer's declaration under rule 24f-2(a)(1), if
         applicable (see instruction A.6):



7.       Number and amount of  securities  of the same class or series which had
         been registered under the Securities Act of 1933 other than pursuant to
         rule 24f-2 in a prior fiscal  year,  but which  remained  unsold at the
         beginning of the fiscal year:

         None

8.       Number and amount of securities registered during the fiscal year other
         than pursuant to rule 24f-2:

         54,002,967 shares for $1,353,675,147

9.       Number and aggregate sale price of securities sold during the fiscal 
         year:

         3,945,103,610 shares for $9,481,643,397.81

10.      Number and aggregate sale price of securities sold during the fiscal 
         year in reliance upon registration pursuant to rule 24f-2:

         3,891,100,643 shares for $8,127,968,250.81

11.      Number and aggregate sale price of securities  issued during the fiscal
         year in connection with dividend reinvestment plans, if applicable (see
         instruction B.7):

         45,761,434 shares for $454,165,763.21


<PAGE>

<TABLE>
<CAPTION>
12.      Calculation of registration fee:

<S>      <C>      <C>                                                                                              <C>
         (i)      Aggregate sale price of securities sold during the fiscal
                  year in reliance on rule 24f-2 (from Item 10):                                                   $8,127,968,250.81

         (ii)     Aggregate price of shares issued in connection with
                  dividend reinvestment plans (from Item 11, if applicable):                                         +454,165,763.21

         (iii)    Aggregate price of shares redeemed or repurchased during
                  the fiscal year (if applicable):                                                                 -7,580,954,727.55

         (iv)     Aggregate price of shares redeemed or repurchased and
                  previously applied as a reduction to filing fees pursuant to
                  rule 24e-2 (if applicable):                                                                        +             0

         (v)      Net aggregate price of securities sold and issued during
                  the fiscal year in reliance on rule 24f-2 [line (i), plus line
                  (ii), less line (iii), plus line (iv)] (if applicable):                                           1,001,179,286.47

         (vi)     Multiplier prescribed by Section 6(b) of the Securities Act
                  of 1933 or other applicable law or regulation (see
                  instruction C.6):                                                                                x          1/5000

         (vii)    Fee due [line (i) or line (v) multiplied by line (vi)]:                                                 200,235.86
                                                                                                                          ==========
</TABLE>

Instruction:   Issuers should complete lines (ii), (iii),  (iv), and (v) only if
               the form is being  filed  within  60 days  after the close of the
               issuer's fiscal year. See Instruction C.3.


13.      Check  box if fees  are  being  remitted  to the  Commission's  lockbox
         depository  as  described  in section 3a of the  Commission's  Rules of
         Informal and Other Procedures (17 CFR 202.3a).

                                                                             [X]

         Date of mailing or wire  transfer  of filing  fees to the  Commission's
         lockbox depository:

         November 17, 1995


<PAGE>

                                   SIGNATURES

         This report has been signed below by the following persons on behalf of
the issuer and in the capacities and on the dates indicated.

         By (Signature and Title)                /s/ Deborah E. Bielicke
                                                 Name: Deborah E. Bielicke
                                                 Title: Assistant Vice President

         Date: November 17, 1995





                                                                       EXHIBIT 5

JANUS FUNDS
P.O. Box 173375
Denver, Colorado  80217-3375
800/525-3713



November 16, 1995


Janus Investment Fund
100 Fillmore Street, Suite 300
Denver, CO  80206-4923

         Re:  Public Offering of Janus Investment Fund Shares

Gentlemen:

     I have  acted  as  counsel  for  Janus  Investment  Fund  (the  "Fund"),  a
Massachusetts  business trust, in connection with the filing with the Securities
and Exchange  Commission of the Fund's "Rule 24f-2  Notice"  covering the Fund's
issuance of  3,945,103,610  shares of beneficial  interest,  par value $0.01 per
share (the "Issued Shares"), for the Fiscal Year ended October 31, 1995.

     I have examined the Fund's  Agreement and  Declaration of Trust and Bylaws,
as amended,  the  proceedings  of its  Trustees  relating to the  authorization,
issuance  and proposed  sale of the Issued  Shares,  and such other  records and
documents as I have deemed relevant.

     Based upon such  examination,  it is my opinion  that upon the issuance and
sale of the  3,945,103,610  Issued  Shares of Janus  Investment  Fund during the
Fiscal Year ended October 31, 1995, in reliance  upon  registration  pursuant to
Rule  24f-2 and the manner  contemplated  by the  Fund's  1933 Act  Registration
Statement,  as  amended,  such  shares  were  legally  issued,  fully  paid  and
nonassessable.

     I hereby  consent to the  filing of this  opinion as an exhibit to the Rule
24f-2 Notice  referred to above.  This opinion is for the  exclusive  use of the
Fund in  connection  with the filing of the Rule 24f-2 Notice  referred to above
with the  Securities  and  Exchange  Commission  (and certain  state  securities
commissions) and is not to be used, circulated, quoted, relied upon or otherwise
referred to by any other person or for any other purpose.

     This  opinion is given as of the date  hereof  and I render no opinion  and
disclaim any  obligation  to revise or  supplement  this opinion  based upon any
change  in  applicable  law or any  factual  matter  that  occurs or comes to my
attention after the date hereof.

                                Very truly yours,


                                /s/ David C. Tucker
                                David C. Tucker
                                Vice President & General Counsel




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