JANUS INVESTMENT FUND
24F-2NT, 1996-12-13
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                     U.S. SECURITIES AND EXCHANGE COMMISSION
                             Washington, D.C. 20549

                                   FORM 24F-2
                        ANNUAL NOTICE OF SECURITIES SOLD
                             PURSUANT TO RULE 24F-2


1.   Name and address of issuer:

     Janus Investment Fund
     100 Fillmore Street
     Denver, CO 80206-4928

2.   Name of each series or class of funds for which this notice is filed:

     Janus  Government  Money  Market Fund  (Investor  Shares and  Institutional
        Shares) 
     Janus Money Market Fund (Investor  Shares and  Institutional  Shares) 
     Janus  Tax-Exempt  Money  Market Fund  (Investor  Shares and  Institutional
        Shares) 
     Janus Balanced Fund 
     Janus Enterprise Fund 
     Janus Equity Income Fund
     Janus Federal Tax-Exempt Fund 
     Janus Flexible Income Fund 
     Janus Fund  
     Janus Growth and Income Fund 
     Janus High-Yield Fund 
     Janus Intermediate Government Securities Fund 
     Janus Mercury Fund 
     Janus Olympus Fund 
     Janus Overseas Fund 
     Janus Short-Term Bond Fund 
     Janus Twenty Fund 
     Janus Venture Fund 
     Janus Worldwide Fund

3.   Investment Company Act File Number:  811-1879     

     Securities Act File Number:  2-34393

4.   Last day of fiscal year for which this notice is filed:

     October 31, 1996


<PAGE>


5.   Check box if this  notice is being filed more than 180 days after the close
     of the issuer's fiscal year for purposes of reporting securities sold after
     the close of the fiscal year but before  termination  of the issuer's 24f-2
     declaration:

                                                                      [  ]

6.   Date of  termination of issuer's  declaration  under rule  24f-2(a)(1),  if
     applicable (see instruction A.6):



7.   Number and amount of  securities of the same class or series which had been
     registered  under the  Securities  Act of 1933 other than  pursuant to rule
     24f-2 in a prior fiscal year, but which remained unsold at the beginning of
     the fiscal year:

     None

8.   Number and amount of  securities  registered  during the fiscal  year other
     than pursuant to rule 24f-2:

     None

9.   Number and aggregate sale price of securities sold during the fiscal year:

     27,867,839,458.08 shares for $39,137,828,150.15


10.  Number and aggregate  sale price of securities  sold during the fiscal year
     in reliance upon registration pursuant to rule 24f-2:

     27,867,839,458.08 shares for $39,137,828,150.15

11.  Number and aggregate sale price of securities issued during the fiscal year
     in  connection  with  dividend   reinvestment  plans,  if  applicable  (see
     instruction B.7):

     135,751,347.385 shares for $1,841,119,692.32


<PAGE>


12.  Calculation of registration fee:

<TABLE>
<S>                                                                             <C>   
     (i)    Aggregate sale price of securities sold during the 
            fiscal year in reliance on rule 24f-2 (from Item 10):               $39,137,828,150.15
                                                                                 _________________

     (ii)   Aggregate  price of shares  issued in  connection  with  
            dividend reinvestment plans (from Item 11, if applicable):          + 1,841,119,692.32
                                                                                  ________________

     (iii)  Aggregate price of shares redeemed or repurchased 
            during the fiscal year (if applicable):                             -32,915,554,587.24
                                                                                  ________________

     (iv)   Aggregate price of shares redeemed or repurchased and
            previously applied as a reduction to filing fees pursuant 
            to rule 24e-2 (if applicable):                                      +         0
                                                                                  _________________

     (v)    Net aggregate price of securities sold and issued during
            the fiscal year in reliance on rule 24f-2 [line (i), plus 
            line (ii), less line (iii), plus line (iv)] (if applicable):           8,063,393,255.20
                                                                                 __________________

     (vi)   Multiplier prescribed by Section 6(b) of the Securities Act
            of 1933 or other applicable law or regulation (see
            instruction C.6):                                                   x       1/3300
                                                                                 __________________

     (vii)  Fee due [line (i) or line (v) multiplied by line (vi)]:                    2,443,452.50
                                                                                ===================
</TABLE>

INSTRUCTION:   ISSUERS SHOULD COMPLETE LINES (II), (III),  (IV), AND (V) ONLY IF
               THE FORM IS BEING  FILED  WITHIN  60 DAYS  AFTER THE CLOSE OF THE
               ISSUER'S FISCAL YEAR. SEE INSTRUCTION C.3.


13.  Check box if fees are being remitted to the Commission's lockbox depository
     as described in section 3a of the Commission's  Rules of Informal and Other
     Procedures (17 CFR 202.3a).

                                                                      [X]

     Date of mailing or wire transfer of filing fees to the Commission's lockbox
     depository:

     December 13, 1996


<PAGE>


                                   SIGNATURES

     This report has been signed below by the following persons on behalf of the
issuer and in the capacities and on the dates indicated.

     By (Signature and Title)    /s/Kelley Abbott Howes

                                 Name:  Kelley Abbott Howes
                                 Title: Secretary

     Date:  December 13, 1996





                                                                       EXHIBIT 5

December 13, 1996


Janus Investment Fund
100 Fillmore Street
Denver, CO  80206-4928

     Re:  Public Offering of Janus Investment Fund Shares

Gentlemen:

     I have  acted  as  counsel  for  Janus  Investment  Fund  (the  "Fund"),  a
Massachusetts  business trust, in connection with the filing with the Securities
and Exchange Commission of the Fund's Form 24F-2 covering the Fund's issuance of
27,867,839,458  shares of  beneficial  interest,  par value $0.01 per share (the
"Issued Shares"), for the Fiscal Year ended October 31, 1996.

     I have examined the Fund's  Agreement and  Declaration of Trust and Bylaws,
as amended,  the  proceedings  of its  Trustees  relating to the  authorization,
issuance  and proposed  sale of the Issued  Shares,  and such other  records and
documents as I have deemed relevant.

     Based upon such  examination,  it is my opinion  that upon the issuance and
sale of the  27,867,839,458  Issued Shares of Janus  Investment  Fund during the
Fiscal Year ended October 31, 1996, in reliance  upon  registration  pursuant to
Rule  24f-2 and the manner  contemplated  by the  Fund's  1933 Act  Registration
Statement,  as  amended,  such  shares  were  legally  issued,  fully  paid  and
nonassessable.

     I hereby  consent to the filing of this opinion as an exhibit to Form 24F-2
referred  to  above.  This  opinion  is for  the  exclusive  use of the  Fund in
connection  with the filing of Form 24F-2  referred to above with the Securities
and Exchange Commission (and certain state securities commissions) and is not to
be used,  circulated,  quoted, relied upon or otherwise referred to by any other
person or for any other purpose.

     This  opinion is given as of the date  hereof  and I render no opinion  and
disclaim any  obligation  to revise or  supplement  this opinion  based upon any
change  in  applicable  law or any  factual  matter  that  occurs or comes to my
attention after the date hereof.

                                        Very truly yours,


                                        /S/David C. Tucker
                                        David C. Tucker
                                        Vice President & General Counsel





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