JANUS INVESTMENT FUND
24F-2NT, 1997-12-19
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                    U.S. SECURITIES AND EXCHANGE COMMISSION
                             Washington, D.C. 20549

                                   FORM 24F-2
                        ANNUAL NOTICE OF SECURITIES SOLD
                             PURSUANT TO RULE 24F-2


1.        Name and address of issuer:

          Janus Investment Fund
          100 Fillmore Street
          Denver, CO 80206-4928


2.        The name of each series or class of securities for which this Form is
          filed (If the Form is being filed for all series and classes of
          securities of the issuer, check the box, but do not list series or
          classes): [X ]


3.        Investment Company Act File Number:  811-1879

          Securities Act File Number:  2-34393


4(a).     Last day of fiscal year for which this Form is filed:

          October 31, 1997


4(b).     [  ]  Check box if this Form is being filed late (i.e., more than 90 
          calendar days after the end of the issuer's fiscal year).  
          (See Instruction A.2)

Note:     If the Form is being filed late, interest must be paid on the
          registration fee due.


4(c).     [ ] Check box if this is the last time the issuer will be filing this
          Form.


<PAGE>


5.   Calculation of registration fee:

     (i)  Aggregate sale price of securities sold during
          the fiscal year pursuant to section  24(f):       $ 88,254,355,500.02
                                                              -----------------


     (ii) Aggregate price of securities redeemed or
          repurchased during the fiscal year:          $ 76,453,351,612.46
                                                         -----------------


     (iii) Aggregate price of securities redeemed or 
           repurchased during any prior fiscal year ending 
           no earlier than October 11, 1995 that were not
           previously used to reduce registration fees payable
           to the Commission:                                      $      0
                                                                    ------------


     (iv) Total available redemption credits [add Items   - $ 76,453,351,612.46
          5(ii) and 5(iii)]:                                  -----------------


     (v)  Net sales - if Item 5(i) is greater than Item 5(iv)
          [subtract Item 5(iv) from Item 5(i)]:              $ 11,801,003,887.56
                                                               -----------------


     (vi) Redemption credits available for use in future     $ (    0   ) 
          years --  if Item 5(i) is less than Item 5(iv)        --------- 
          [subtract Item 5(iv) from Item 5(i)]:


     (vii) Multiplier for determining registration fee (See
           Instruction C.9):                                   x  0.000295
                                                                 --------


     (viii) Registration fee due [multiply Item 5(v) by Item
            5(vii)] (enter "0" if no fee is due):            =$ 3,481,296.15
                                                               ============


6.   Prepaid Shares

     If the response to item 5(i) was determined by deducting an amount of
     securities that were registered under the Securities Act of 1933
     pursuant to rule 24e-2 as in effect before October 11, 1997, then
     report the amount of securities (number of shares or other units)
     deducted here: 0 . If there is a number of shares or other units that
     were registered pursuant to rule 24e-2 remaining unsold at the end of
     the fiscal year for which this form is filed that are available for use
     by the issuer in future fiscal years, then state that number here: 0 .


<PAGE>


7.   Interest due - if this Form is being filed more than 90 days after the end
     of the issuer's fiscal year (see Instruction D):


                                                            +$    0
                                                               ---------


8.   Total of the amount of the registration fee due plus any interest due
     [line 5(viii) plus line 7]:


                                                            =$ 3,481,296.15
                                                               ============
                                                               


9.   Date the registration fee and any interest payment was sent to the
     Commission's lockbox depository: December 19, 1997



     Method of Delivery

                           [ X ]  Wire Transfer
                           [  ]   Mail or other means


<PAGE>


                                   SIGNATURES

This report has been signed below by the following persons on behalf of the
issuer and in the capacities and on the dates indicated.

     By (Signature and Title)      /s/ Kelley Abbott Howes

                                   Name: Kelley Abbott Howes
                                   Title:    Secretary

Date:  December 19, 1997



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