JANUS INVESTMENT FUND
485APOS, EX-99.H, 2000-12-18
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                                                                    EXHIBIT 8(O)

September 26, 2000

Ms. Marjorie G. Hurd
Janus Service Corporation
100 Fillmore Street
Denver, Colorado 80206

Dear Ms. Hurd:

Attached are revised  Appendix A and Appendix B to the Transfer Agency Agreement
dated as of September 27, 1995 (the "Agreement"),  between Janus Investment Fund
(the "Trust") and Janus Service Corporation ("JSC"). The revised Appendices will
be effective as of September  26, 2000.  The purpose of the  revisions is to add
Janus Fund 2 as an additional  portfolio of the Trust.  Pursuant to Section 9 of
the Agreement,  the Fund hereby  requests that JSC acknowledge its acceptance of
the terms contained in each of the revised Appendices.

Please indicate your acceptance of the foregoing by executing two copies of this
letter, returning one copy to the Trust and retaining one copy for your records.

JANUS INVESTMENT FUND

BY:   /s/ Kelley Abbott Howes
      Kelley Abbott Howes
      Vice President

JANUS SERVICE CORPORATION

BY:   /s/ Marjorie G. Hurd
      Marjorie G. Hurd
      President

Agreed to this 26th day of September, 2000

cc:      Suzanne F. Olczak
         Christine A. Scheel


<PAGE>


                                                Revised as of September 26, 2000

                                   APPENDIX A

Janus Money Market Fund
Janus Government Money Market Fund
Janus Tax-Exempt Money Market Fund
Janus High-Yield Fund
Janus Olympus Fund
Janus Venture Fund
Janus Fund
Janus Twenty Fund
Janus Enterprise Fund
Janus Mercury Fund
Janus Overseas Fund
Janus Worldwide Fund
Janus Growth and Income Fund
Janus Balanced Fund
Janus Flexible Income Fund
Janus Short-Term Bond Fund
Janus Federal Tax-Exempt Fund
Janus Equity Income Fund
Janus Special Situations Fund
Janus Global Life Sciences Fund
Janus Global Technology Fund
Janus Strategic Value Fund
Janus Orion Fund
Janus Fund 2


<PAGE>


                                                Revised as of September 26, 2000

                                   APPENDIX B

I.       NON-MONEY MARKET PORTFOLIOS

Each  non-money  market  portfolio  of the Trust shall pay JSC for its  transfer
agency  services a fee,  calculated and payable for each day that this Agreement
is in effect,  of 1/365 of 0.16% of the daily  closing  net asset  value of such
portfolio,  plus reasonable  out-of-pocket  expenses incurred in connection with
JSC's  services as transfer  agent.  In addition,  each of the non-money  market
portfolios  listed below shall pay a monthly fee at the annual rate of $4.00 per
open shareholder account per year:

                               Janus Balanced Fund
                              Janus Enterprise Fund
                            Janus Equity Income Fund
                          Janus Federal Tax-Exempt Fund
                           Janus Flexible Income Fund
                                  Janus Fund 2
                         Janus Global Life Sciences Fund
                          Janus Global Technology Fund
                          Janus Growth and Income Fund
                              Janus High-Yield Fund
                               Janus Mercury Fund
                               Janus Olympus Fund
                                Janus Orion Fund
                           Janus Short-Term Bond Fund
                          Janus Special Situations Fund
                           Janus Strategic Value Fund
                              Janus Worldwide Fund

All such fees shall be subject to reduction as set forth in section 5.c. of this
Agreement.  If an account is open on any day of a month, the per account fee (if
applicable) shall be payable for that month.

II.      MONEY MARKET PORTFOLIOS

Notwithstanding  the above,  however,  JSC agrees  that it shall not look to the
Funds or the  Trust  for  compensation  for its  services  provided  under  this
Agreement  to Janus Money  Market Fund,  Janus  Government  Money Market Fund or
Janus Tax-Exempt Money Market Fund (collectively,  the "Money Funds"). JSC shall
be  compensated  for its services to the Money Funds  entirely by Janus  Capital
Corporation, the administrator to the Money Funds, pursuant to an Administration
Agreement between Janus Capital Corporation and each of the Money Funds.



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