JANUS INVESTMENT FUND
497, 2000-05-09
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                        Janus Global Life Sciences Fund

                         Supplement dated May 10, 2000
                     to Prospectus dated January 31, 2000,
                         as supplemented March 16, 2000

THIS INFORMATION SUPPLEMENTS THE PROSPECTUS DATED JANUARY 31, 2000, AS
SUPPLEMENTED MARCH 16, 2000. THIS SUPPLEMENT AND THE PROSPECTUS CONSTITUTE A
CURRENT PROSPECTUS. TO REQUEST ANOTHER COPY OF THE PROSPECTUS, PLEASE CALL
1-800-525-3713.

Janus Global Life Sciences Fund (the "Fund") is closed to new investors
effective May 10, 2000. Purchases into new accounts will be accepted only
through the close of business on May 9, 2000. After May 9, 2000, purchases into
new accounts generally will no longer be accepted.

After May 9, 2000, the following may continue to make additional purchases and
to reinvest dividends and capital gains into existing accounts:

a. Current Fund shareholders (additionally, current Fund shareholders may open
   new accounts in the Fund);

b. Discretionary investment advisers that invest through existing accounts at a
   financial institution; however, after the close of business on May 9, 2000,
   no investments in the Fund will be accepted for any new accounts even if they
   will be established through an existing relationship with a discretionary
   investment adviser; and

c. Participants in qualified defined contribution retirement plans (for example,
   401(k) plans, profit sharing plans and money purchase pension plans), 403(b)
   plans and 457 plans that invest through existing omnibus accounts at a
   financial institution.

Once an account is closed, additional investments may not be accepted.

Investors who do not have open accounts in the Fund as of the close of business
on May 9, 2000 will not be allowed to purchase shares of the Fund, except that:

a. Employees of Janus Capital Corporation or its subsidiaries may open new
   accounts directly with Janus; and

b. Certain defined contribution plans that have selected Janus as part of their
   investment program, but have not established an account, may open new
   accounts in the Fund as long as their account is funded with Janus by January
   31, 2001.

                                                             (Continued on back)
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Except as otherwise noted, these restrictions apply to investments made directly
with Janus and investments made through financial institutions. Investors may be
required to demonstrate eligibility to purchase shares of the Fund before an
investment is accepted. The Fund may resume sales of shares to new investors at
some future date, but it has no present intention to do so.

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