JANUS INVESTMENT FUND
497, 2000-05-09
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                               Janus Equity Funds

                         Supplement dated May 10, 2000
                     to Prospectus dated January 31, 2000,
                         as supplemented March 16, 2000

THIS INFORMATION SUPPLEMENTS THE PROSPECTUS DATED JANUARY 31, 2000, AS
SUPPLEMENTED MARCH 16, 2000. THIS SUPPLEMENT AND THE PROSPECTUS CONSTITUTE A
CURRENT PROSPECTUS. TO REQUEST ANOTHER COPY OF THE PROSPECTUS, PLEASE CALL
1-800-525-3713.

Janus Olympus Fund, Janus Global Life Sciences Fund and Janus Worldwide Fund
(individually, the "Fund" and collectively, the "Funds") are closed to new
investors effective May 10, 2000. Purchases into new accounts will be accepted
only through the close of business on May 9, 2000. After May 9, 2000, purchases
into new accounts generally will no longer be accepted.

After May 9, 2000, the following may continue to make additional purchases and
to reinvest dividends and capital gains into existing accounts:

a. Current shareholders of the Funds (additionally, current shareholders of the
   Funds may open new accounts in any Fund in which they currently own shares);

b. For Janus Global Life Sciences Fund and Janus Worldwide Fund, discretionary
   investment advisers that invest through existing accounts at a financial
   institution; however, after the close of business on May 9, 2000, no
   investments in Janus Global Life Sciences Fund and Janus Worldwide Fund will
   be accepted for any new accounts even if they will be established through an
   existing relationship with a discretionary investment adviser;

c. For Janus Olympus Fund, discretionary investment advisers that invest through
   existing accounts at a financial institution (additionally, discretionary
   investment advisers that have existing accounts in the Fund may establish new
   accounts in the Fund);

d. For Janus Global Life Sciences Fund and Janus Worldwide Fund, participants in
   qualified defined contribution retirement plans (for example, 401(k) plans,
   profit sharing plans and money purchase pension plans), 403(b) plans and 457
   plans that invest through existing omnibus accounts at a financial
   institution; and

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e. For Janus Olympus Fund, financial institutions that currently have a
   contractual agreement with Janus to offer the Fund to qualified defined
   contribution retirement plans and their participants (for example, 401(k)
   plans, profit sharing plans and money purchase pension plans), 403(b) plans
   and 457 plans.

Once an account is closed, additional investments may not be accepted.

Investors who do not have open accounts in a Fund as of the close of business on
May 9, 2000 will not be allowed to purchase shares of a Fund, except that:

a. Employees of Janus Capital Corporation or its subsidiaries may open new
   accounts directly with Janus; and

b. For Janus Global Life Sciences Fund and Janus Worldwide Fund, certain defined
   contribution plans that have selected Janus as part of their investment
   program, but have not established an account, may open new accounts in a Fund
   as long as their account is funded with Janus by January 31, 2001.

Except as otherwise noted, these restrictions apply to investments made directly
with Janus and investments made through financial institutions. Investors may be
required to demonstrate eligibility to purchase shares of a Fund before an
investment is accepted. A Fund may resume sales of shares to new investors at
some future date, but there is no present intention to do so.

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