APPLIED IMAGING CORP
SC 13G, 1998-02-12
SURGICAL & MEDICAL INSTRUMENTS & APPARATUS
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                         UNITED STATES
              SECURITIES AND EXCHANGE COMMISSION
                    Washington, D.C.  20549


                          SCHEDULE 13G


            Under the Securities Exchange Act of 1934
                    (Amendment No. _____)*


                         Applied Imaging 
      ______________________________________________________
                        (Name of Issuer)

                    Common stock,  No Par Value   
      ______________________________________________________
                 (Title of Class of Securities)

                         03820G106               
               ____________________________________
                         (CUSIP Number)


Check the following box if a fee is being paid with this
statement |  |.  (A fee is not required only if the filing
person: (1) has a previous statement on file reporting beneficial
ownership of more than five percent of the class of securities
described in Item 1; and (2) has filed no amendment subsequent
thereto reporting beneficial ownership of five percent or less of
such class).  (See Rule 13d-7).

*The remainder of this cover page shall be filled out for a
reporting person's initial filing on this form with respect to
the subject class of securities, and for any subsequent amendment
containing information which would alter the disclosures provided
in a prior cover page.

The information required in the remainder of the cover page shall
not be deemed to be "filed" for the purpose of Section 18 of the
Securities Exchange Act of 1934 ("Act") or otherwise subject to
the liabilities of that section of the Act but shall be subject
to all other provisions of the Act (however, see the Notes).










CUSIP No.03820G106               13G         Page  2  of  12

- ----------------------------------------------------------------
(1)  NAME OF REPORTING PERSON
     S.S. OR I.R.S. IDENTIFICATION NO. OF ABOVE PERSON

     Special Situations Fund III, L.P. ("The Fund")
     13-3737427
- ----------------------------------------------------------------
(2)  CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP*  (a) | |
                                                        (b) |X|
- ----------------------------------------------------------------
(3)  SEC USE ONLY

- ----------------------------------------------------------------
(4)  CITIZENSHIP OR PLACE OF ORGANIZATION

     Delaware
- ----------------------------------------------------------------
 NUMBER OF     (5) SOLE VOTING POWER             494,800
  SHARES       -------------------------------------------------
BENEFICIALLY   (6) SHARED VOTING POWER           None
 OWNED BY      -------------------------------------------------
   EACH        (7) SOLE DISPOSITIVE POWER         494,800
 REPORTING     -------------------------------------------------
PERSON WITH    (8) SHARED DISPOSITIVE POWER      None
- ----------------------------------------------------------------
(9)  AGGREGATE AMOUNT BENEFICALLY OWNED BY EACH REPORTING PERSON

     494,800
- ----------------------------------------------------------------
(10) CHECK BOX IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES
     CERTAIN SHARES*

- ----------------------------------------------------------------
(11) PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW 9

     6.5
- ----------------------------------------------------------------
(12) TYPE OF REPORTING PERSON*

     IA
- ----------------------------------------------------------------











CUSIP No.03820G106               13G         Page  3  of 12

- ----------------------------------------------------------------
(1)  NAME OF REPORTING PERSON
     S.S. OR I.R.S. IDENTIFICATION NO. OF ABOVE PERSON

     MGP Advisers Limited Partnership, L.P. ("MGP")
     13-3263120
- ----------------------------------------------------------------
(2)  CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP*  (a) | |
                                                        (b) |X|
- ----------------------------------------------------------------
(3)  SEC USE ONLY

- ----------------------------------------------------------------
(4)  CITIZENSHIP OR PLACE OF ORGANIZATION

     Delaware
- ----------------------------------------------------------------
 NUMBER OF     (5) SOLE VOTING POWER             None
  SHARES       -------------------------------------------------
BENEFICIALLY   (6) SHARED VOTING POWER           None
 OWNED BY      -------------------------------------------------
   EACH        (7) SOLE DISPOSITIVE POWER         494,800
 REPORTING     -------------------------------------------------
PERSON WITH    (8) SHARED DISPOSITIVE POWER      None
- ----------------------------------------------------------------
(9)  AGGREGATE AMOUNT BENEFICALLY OWNED BY EACH REPORTING PERSON

     494,800
- ----------------------------------------------------------------
(10) CHECK BOX IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES
     CERTAIN SHARES*

- ----------------------------------------------------------------
(11) PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW 9

     6.5
- ----------------------------------------------------------------
(12) TYPE OF REPORTING PERSON*

     IA
- ----------------------------------------------------------------











CUSIP No.03820G106               13G         Page  4  of  12

- ----------------------------------------------------------------
(1)  NAME OF REPORTING PERSON
     S.S. OR I.R.S. IDENTIFICATION NO. OF ABOVE PERSON

     AWM Investment Company, Inc. ("AWM")
     11-2086452
- ----------------------------------------------------------------
(2)  CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP*  (a) | |
                                                        (b) |X|
- ----------------------------------------------------------------
(3)  SEC USE ONLY

- ----------------------------------------------------------------
(4)  CITIZENSHIP OR PLACE OF ORGANIZATION

     Delaware
- ----------------------------------------------------------------
 NUMBER OF     (5) SOLE VOTING POWER             166,100
  SHARES       -------------------------------------------------
BENEFICIALLY   (6) SHARED VOTING POWER           None
 OWNED BY      -------------------------------------------------
   EACH        (7) SOLE DISPOSITIVE POWER         660,900
 REPORTING     -------------------------------------------------
PERSON WITH    (8) SHARED DISPOSITIVE POWER      None
- ----------------------------------------------------------------
(9)  AGGREGATE AMOUNT BENEFICALLY OWNED BY EACH REPORTING PERSON

     660,900
- ----------------------------------------------------------------
(10) CHECK BOX IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES
     CERTAIN SHARES*

- ----------------------------------------------------------------
(11) PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW 9

     8.6
- ----------------------------------------------------------------
(12) TYPE OF REPORTING PERSON*

     IA
- ----------------------------------------------------------------











CUSIP No.03820G106              13G         Page  5   of  12

- ----------------------------------------------------------------
(1)  NAME OF REPORTING PERSON
     S.S. OR I.R.S. IDENTIFICATION NO. OF ABOVE PERSON
     
     Austin W. Marxe
     ###-##-####
- ----------------------------------------------------------------
(2)  CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP*  (a) | |
                                                        (b) |X|
- ----------------------------------------------------------------
(3)  SEC USE ONLY

- ----------------------------------------------------------------
(4)  CITIZENSHIP OR PLACE OF ORGANIZATION
   
     United States
- ----------------------------------------------------------------
 NUMBER OF     (5) SOLE VOTING POWER         166,100
  SHARES       -------------------------------------------------
BENEFICIALLY   (6) SHARED VOTING POWER       494,800
 OWNED BY      -------------------------------------------------
   EACH        (7) SOLE DISPOSITIVE POWER    660,900
 REPORTING     -------------------------------------------------
PERSON WITH    (8) SHARED DISPOSITIVE POWER       None
- ----------------------------------------------------------------
(9)  AGGREGATE AMOUNT BENEFICALLY OWNED BY EACH REPORTING PERSON

     660,900
- ----------------------------------------------------------------
(10) CHECK BOX IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES
     CERTAIN SHARES*

- ----------------------------------------------------------------
(11) PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW 9

     8.6
- ----------------------------------------------------------------
(12) TYPE OF REPORTING PERSON*

     IN 
- ----------------------------------------------------------------











                                             Page 6 of 12 Pages

Item 1.
(a)  Name of Issuer:  Applied Imaging 
(b)  Address of Issuer's Principal Executive Offices:
     2380 Walsh Ave, Bldg B, Santa Clara CA  95051
Item 2.
(a)-(c)Name of Person Filing; Address of Principal Business
Office; and Place of Organization:  This statement is filed on
behalf of (i) Special Situations Fund III, L.P., a Delaware
limited partnership (the "Fund"), (ii) MGP Advisers Limited
Partnership, a Delaware Limited Partnership ("MGP"), (iii) AWM
Investment Company, Inc., a Delaware corporation ("AWM") and
(iv) Austin W. Marxe.  Each of the foregoing is hereinafter
individually referred to as a "Reporting Person" and
collectively as the "Reporting Persons."  The principal office
and business address of the Reporting Persons is 153 East 53
Street, New York, New York 10022.  The business of the Fund is
to acquire, purchase, invest in, sell, convey, transfer,
exchange and otherwise trade in principally equity and equity
related securities.
MGP is a general partner of and investment adviser to the
Fund.  MGP is registered as an investment adviser under the
Investment Advisers Act of 1940, as amended.  The principal
business of MGP is to act as a general partner of and
investment adviser to the Fund.  AWM, a Delaware corporation
primarily owned by Austin W. Marxe, serves as the sole general
partner of MGP.  AWM is a registered investment adviser under
                                        Page 7 of 12 Pages
 the Investment Advisers Act of 1940 and also serves as the
investment adviser to, and general partner of, Special
Situations Cayman Fund, L.P., a limited partnership formed
under the laws of the Cayman Islands (the "Cayman Fund"). 
Austin W. Marxe is the principal limited partner of MGP and is
the President and Chief Executive Officer of AWM.  Mr. Marxe
is also an associated person of MGP and is principally
responsible for the selection, acquisition and disposition of
the portfolio securities by AWM on behalf of MGP, the Fund and
the Cayman Fund.  
2(b)      Title of Class of Securities: See cover sheets.
2(c)      CUSIP Number:  See cover sheets.
Item 3.   If this statement is filed pursuant to Rule 13d-
          1(b), or 13d-2(b), check whether the person filing
          is a:
(a) ( )   Broker or Dealer registered under section 15 of the
          Act
(b) ( )   Bank as defined in section 3(a) (6) of the Act
(c) ( )   Insurance Company as defined in section 3(a) (19) of
          the Act
(d) (x)   Investment Company registered under section 8 of the
          Investment Company Act 
(e) (x)   Investment Adviser registered under section 203 of
          the Investment Advisers Act of 1940
(f) ( )   Employee Benefit Plan, Pension Fund which is subject
          to the provisions of the Employee Retirement Income
                                        Page 8 of 12 Pages
          Security Act of 1974 or Endowment Fund
(g) (x)   Parent Holding Company, in accordance with $240.13d-
          1 (b) (ii) (G)
(h) ( )   Group, in accordance with $240.13d-1 (b) (1) (ii)
          (H)
See Exhibit A attached hereto.
Item 4.   Ownership:
(a)  Amount Beneficially Owned:  660.900 common shares are
beneficially Austin W. Marxe and AWM.  This amount is composed
of 494,800 shares owned by the Fund, and 166,100 shares owned
by the Cayman Fund.  The Fund and MGP beneficially own 494,800
shares.
(b)  Percent of Class:  8.6 percent are beneficially owned by
Austin W. Marxe and AWM.  6.5 percent are beneficially owned
by the Fund and MGP.   
(c)  Number of Shares as to Which Such Person Has Rights to 
Vote and/or Dispose of Securities: The Special Situations Fund
III, L.P. has the sole power to vote or to direct the vote and
to dispose or to direct the disposition of all securities
reported hereby which are beneficially owned by the Fund.  AWM
has the sole power to vote or to direct the vote of securities
reported hereby which are benefically owned by AWM by virtue
of it being the Investment Adviser to the Cayman Fund.  Austin
Marxe has sole power to vote or to direct the vote of
securities reported hereby which are beneficially owned by
Austin Marxe by virtue of being the primary owner of and
                              Page 9 of 12 Pages
 President and Chief Executive Officer of AWM.  Austin Marxe
has shared power to vote or to direct the vote of all
securities owned by Austin Marxe by virtue of being an
Individual General Partner of the Fund.  MGP, AWM and Austin
Marxe have the sole power to dispose or to direct the
disposition of all securities reported hereby which are
beneficially owned respectively by MGP, AWM and Austin Marxe.
Item 5.   Ownership of Five Percent or Less of a Class:
     If this statement is being filed to report the fact that
as of the date hereof the reporting person has ceased to be
the beneficial owner of more that five percent of the class of
securities, check the following  x .
Item 6.   Ownership of More than Five Percent on Behalf of
 Another Person:  The Fund and the Cayman Fund as owners of
the securities in question, have the right to receive any
dividends from, or proceeds from the sale of, such securities. 
Item 7.   Identification and Classification of the Subsidiary
 Which Acquired the Security being Reported on by the Parent
Holding Company:  See Exhibit A attached hereto.
Item 8.   Identification and Classification of Members of the 
          Group:  Not applicable.
Item 9.   Notices of Dissolution of Group:  Not applicable.




                              Page 10 of 12 PAges
Item 10.  Certification:
               Each of the undersigned certifies that, to the
     best of its or his knowledge and belief, the securities
     referred to above were acquired in the ordinary course of
     business and were acquired for the purpose of and do not
     have the effect of changing or influencing the control of
     the  issuer of such securities and were not acquired in
     connection with or as a participant in any transaction
     having such purpose or effect.

















                              Page 11 of 12 Pages

                          SIGNATURE

     After reasonable inquiry and to the best of his knowledge
and belief, each of the undersigned certifies that the
information set forth in this statement is true, complete and
correct.
Date: February 2, 1998
                    
                         SPECIAL SITUATIONS FUND III, L.P.

                         By:/s/ Austin W. Marxe        
                            Austin W. Marxe
                            Individual General Partner

                         MGP ADVISERS LIMITED PARTNERSHIP
                         By: AWM Investment Company, Inc,



                         By: /s/ Austin W. Marxe        
                             Austin W. Marxe
                             President and Chief              
                             Executive Officer

                              AWM INVESTMENT COMPANY, INC.



                         By: /s/ Austin W. Marxe        
                             Austin W. Marxe
                             President and Chief              
                             Executive Officer


                              /s/ Austin W. Marxe            
                              AUSTIN W. MARXE





                                        Page 12 of 12 Pages




                          EXHIBIT A


     This Exhibit explains the relationship between the
Reporting Persons.  AWM is the sole general partner of MGP, a
registered investment adviser under the Investment Advisers
Act of 1940, as amended.  MGP is a general partner of and
investment adviser to the Fund.  AWM is a registered
investment adviser under the Investment Advisers Act of 1940
and also serves as the general partner of, and investment
adviser to, the Cayman Fund.  Austin W. Marxe is the principal
owner and President of AWM.





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