PERSEPTIVE BIOSYSTEMS INC
S-8, 1997-03-21
LABORATORY ANALYTICAL INSTRUMENTS
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<PAGE>
 
    As filed with the Securities and Exchange Commission on March 21, 1997.
                                      Registration No. 333-
================================================================================


                                        
                       SECURITIES AND EXCHANGE COMMISSION
                            WASHINGTON, D.C.  20549

                                    FORM S-8

                          REGISTRATION STATEMENT UNDER
                           THE SECURITIES ACT OF 1933

                          PerSeptive Biosystems, Inc.
             (Exact name of registrant as specified in its charter)

      
           Delaware                                           04-2987616
  (State or other jurisdiction of                        (I.R.S. Employer
   incorporation or organization)                       Identification No.)

       500 Old Connecticut Path, Framingham, MA                  01701
        (Address of Principal Executive Offices)              (Zip Code)
                              ____________________

                                1992 Stock Plan
                            (Full title of the plan)
                              ____________________

                                Noubar B. Afeyan
                            Chief Executive Officer
                          PerSeptive Biosystems, Inc.
                500 Old Connecticut Path, Framingham, MA   01701
                    (Name and address of agent for service)
                                 (508) 383-7700
         (Telephone number, including area code, of agent for service)
                              ____________________

                                    Copy to:

                            Samuel P. Hunt III, Esq.
                          PerSeptive Biosystems, Inc.
                500 Old Connecticut Path, Framingham, MA   01701
                                 (508) 383-7700


================================================================================
<PAGE>
 
================================================================================
                                        
                        CALCULATION OF REGISTRATION FEE
<TABLE>
<CAPTION>
 
 
                                       Proposed     Proposed
                                       maximum      maximum
Title of               Amount          offering     aggregate       Amount of
securities to          to be           price        offering        registration
be registered          registered      per share    price           fee
- ------------------  ----------------  -----------  -------------    ------------
<S>               <C>                <C>           <C>               <C>
 
Common Stock        286,000 shares     $7.625(1)    $2,180,750.00(1)   $  660.83
(Par Value $.01)    110,400 shares     $6.625(1)    $  731,400.00(1)   $  221.64
                     43,550 shares     $6.375(1)    $  277,631.25(1)   $   84.13
                     73,825 shares     $6.500(1)    $  479,862.50(1)   $  145.41
                    486,225 shares     $8.375(2)    $4,072,134.38(2)   $1,233.98
                  ---------                                            ---------
Total             1,000,000                                            $2,345.99
</TABLE>


================================================================================

    (1) Such shares are issuable upon exercise of outstanding options with fixed
exercise prices. Pursuant to Rule 457(h), the aggregate offering price and the
fee have been computed upon the basis of the price at which such options may be
exercised.

    (2) Estimated solely for the purpose of calculating the registration fee 
pursuant to Rule 457(c) based on the average of the high and low prices reported
on the Nasdaq National Market on March 14, 1997.
  
================================================================================
<PAGE>
 
  This Registration Statement registers additional securities of the same class
as other securities for which Registration Statement No. 33-49642 on Form S-8 as
filed with the Securities and Exchange Commission on July 15, 1992, Registration
Statement No. 33-80856 on Form S-8 as filed with the Securities and Exchange
Commission on June 27, 1994, Registration Statement No. 33-94606 as filed with
the Securities and Exchange Commission on July 14, 1995 and Registration
Statement No. 333-8151 on Form S-8 as filed with the Securities and Exchange
Commission on July 15, 1996 relating to the PerSeptive Biosystems, Inc. 1992
Stock Plan, as amended, and, in the case of the 1992 Registration Statement, the
1989 Stock Plan, the 1992 Non-Employee Director Stock Option Plan, as amended,
and the 1992 Employee Stock Purchase Plan are effective.  Pursuant to General
Instruction E, the contents of the above-listed Registration Statements are
hereby incorporated by reference.


                                    PART II

               INFORMATION REQUIRED IN THE REGISTRATION STATEMENT

Item 8.  Exhibits.
         -------- 

<TABLE> 
<CAPTION> 
       Exhibit No.     Description of Exhibit
       -----------     ----------------------
<S>                    <C>        
       *4.1            1992 Stock Plan of the Registrant, as amended on January 20, 1997.
                       
       *5.1            Opinion of Testa, Hurwitz & Thibeault, LLP.
                       
       *23.1           Consent of Coopers & Lybrand L.L.P.
                       
       *23.2           Consent of Price Waterhouse LLP.
                       
       *23.3           Consent of Testa, Hurwitz & Thibeault, LLP (contained in its
                       opinion as Exhibit 5.1).
                       
       *24.1           Power of Attorney (contained in the signature page of this
                       Registration Statement).
</TABLE> 

- --------------------------
* Filed herewith.
<PAGE>
 
                                  SIGNATURES


    Pursuant to the requirements of the Securities Act of 1933, the Registrant
certifies that it has reasonable grounds to believe that it meets all of the
requirements for filing on Form S-8 and has duly caused this Registration
Statement to be signed on its behalf by the undersigned, thereunto duly
authorized, in the City of Framingham and the Commonwealth of Massachusetts, on
this 21st day of March, 1997.


                                    PERSEPTIVE BIOSYSTEMS, INC.


                                    By:   /s/ Noubar B. Afeyan
                                       -----------------------
                                      Noubar B. Afeyan
                                      Chief Executive Officer

    We, the undersigned officers and directors of PerSeptive Biosystems, Inc.
hereby severally constitute and appoint Noubar B. Afeyan, Thomas G. Ruane and
John F. Smith, and each of them singly, our true and lawful attorneys with
full power to them, and each of them singly, to sign for us and in our names in
the capacities indicated below, the Registration Statement on Form S-8 filed
herewith and any and all amendments (including post-effective amendments) to
said Registration Statement, and generally to do all things in our names and on
our behalf in our capacities as officers and directors to enable PerSeptive
Biosystems, Inc. to comply with the provisions of the Securities Act of 1933, as
amended, and all requirements of the Securities and Exchange Commission, hereby
ratifying and confirming our signatures as they may be signed by our said
attorneys, or any of them, to said Registration Statement and all amendments
thereto.

    Pursuant to the requirements of the Securities Act of 1933, this
Registration Statement has been signed by the following persons in the
capacities and on the date indicated.

Signature                Capacity                              Date
- -----------              --------                              ----
                                                        
                                                        
/s/ Noubar B. Afeyan     Chief Executive                    March 21, 1997
- --------------------     Officer (Principal Executive   
Noubar B. Afeyan         Officer), Director and Chairman
                         of the Board of Directors       
                       


/s/ John F. Smith        President and Director             March 21, 1997
- --------------------
John F. Smith
<PAGE>
 
/s/ Thomas G. Ruane      Senior Vice President and Chief    March 21, 1997
- --------------------     Financial Officer        
Thomas G. Ruane          (Principal Financial and 
                         Accounting Officer)       
                       


/s/ Edwin M. Kania, Jr.  Director                           March 21, 1997
- -----------------------
Edwin M. Kania, Jr.




/s/ Daniel I.C. Wang     Director                           March 21, 1997
- --------------------                                             
Daniel I.C. Wang


/s/ William F. Pounds    Director                           March 21, 1997
- ---------------------
William F. Pounds


/s/ Bruce J. Ryan        Director                           March 21, 1997
- -----------------                                                
Bruce J. Ryan
<PAGE>
 
                                 EXHIBIT INDEX
                                 -------------
<TABLE> 
<CAPTION> 

        Exhibit     Description of Exhibit
        -------     ----------------------
<S>                 <C>                  
         *4.1       1992 Stock Plan of the Registrant, as amended on February 8, 1996.
                 
         *5.1       Opinion of Testa, Hurwitz & Thibeault, LLP.
                 
         *23.1      Consent of Coopers & Lybrand L.L.P.
                    
         *23.2      Consent of Price Waterhouse LLP.
                    
         *23.3      Consent of Testa, Hurwitz & Thibeault, LLP (contained in its opinion as Exhibit 5.1).
                    
         *24.1      Power of Attorney (contained in the signature page of this Registration Statement).
</TABLE> 
     ___________________
     * Filed herewith.

<PAGE>
 
                                                                     EXHIBIT 4.1
                                                                     -----------


                                   (As Amended, January 20, 1997)

                          PERSEPTIVE BIOSYSTEMS, INC.

                                1992 STOCK PLAN
                                ---------------



  1.   Purpose.  This 1992 Stock Plan (the "Plan") is intended to provide
       -------                                                           
incentives: (a) to the officers and other employees of PerSeptive Biosystems,
Inc. (the "Company"), its parent (if any) and any present or future subsidiaries
of the Company (collectively, "Related Corporations") by providing them with
opportunities to purchase stock in the Company pursuant to options granted
hereunder which qualify as "incentive stock options" under Section 422(b) of the
Internal Revenue Code of 1986, as amended (the "Code") ("ISO" or "ISOs"); (b) to
directors, officers, employees and consultants of the Company and Related
Corporations by providing them with opportunities to purchase stock in the
Company pursuant to options granted hereunder which do not qualify as ISOs
("Non-Qualified Option" or "Non-Qualified Options"); (c) to directors, officers,
employees and consultants of the Company and Related Corporations by providing
them with awards of stock in the Company ("Awards"); and (d) to directors,
officers, employees and consultants of the Company and Related Corporations by
providing them with opportunities to make direct purchases of stock in the
Company ("Purchases").  Both ISOs and Non-Qualified Options are referred to
hereafter individually as an "Option" and collectively as "Options".  Options,
Awards and authorizations to make Purchases are referred to hereafter
collectively as "Stock Rights".  As used herein, the terms "parent" and
"subsidiary" mean "parent corporation" and "subsidiary corporation",
respectively, as those terms are defined in Section 424 of the Code.

  2.   Administration of the Plan.
       ---------------------------

       A.   Board or Committee Administration.  The Plan shall be administered
            ---------------------------------                                 
by the Board of Directors of the Company (the "Board") or by a committee
appointed by the Board (the "Committee"); provided, that, to the extent required
by Rule 16b-3, or any successor provision ("Rule 16b-3"), of the Securities
Exchange Act of 1934, with respect to specific grants of Stock Rights, the Plan
shall be administered by a disinterested administrator or administrators within
the meaning of Rule 16b-3.  Hereinafter, all references in this Plan to the
"Committee" shall mean the Board if no Committee has been appointed.  Subject to
ratification of the grant or authorization of each Stock Right by the Board (if
so required by applicable state law), and subject to the terms of the Plan, the
Committee shall have the authority to (i) determine the employees of the Company
and Related Corporations (from among the class of employees eligible under
paragraph 3 to receive ISOs) to whom ISOs may be granted, and to determine (from
among the class of individuals and entities eligible under paragraph 3 to
receive Non-Qualified Options and Awards and to make Purchases) to whom Non-
Qualified Options, Awards
<PAGE>
 
and authorizations to make Purchases may be granted; (ii) determine the time or
times at which Options or Awards may be granted or Purchases made; (iii)
determine the option price of shares subject to each Option, which price shall
not be less than the minimum price specified in paragraph 6, and the purchase
price of shares subject to each Purchase; (iv) determine whether each Option
granted shall be an ISO or a Non-Qualified Option; (v) determine (subject to
paragraph 7) the time or times when each Option shall become exercisable and the
duration of the exercise period; (vi) determine whether restrictions such as
repurchase options are to be imposed on shares subject to Options, Awards and
Purchases and the nature of such restrictions, if any, and (vii) interpret the
Plan and prescribe and rescind rules and regulations relating to it. If the
Committee determines to issue a Non-Qualified Option, it shall take whatever
actions it deems necessary, under Section 422 of the Code and the regulations
promulgated thereunder, to ensure that such Option is not treated as an ISO. The
interpretation and construction by the Committee of any provisions of the Plan
or of any Stock Right granted under it shall be final unless otherwise
determined by the Board. The Committee may from time to time adopt such rules
and regulations for carrying out the Plan as it may deem best. No member of the
Board or the Committee shall be liable for any action or determination made in
good faith with respect to the Plan or any Stock Right granted under it.

       B.   Committee Actions.  The Committee may select one of its members as
            -----------------                                                 
its chairman, and shall hold meetings at such times and places as it may
determine.  Acts by a majority of the Committee, or acts reduced to or approved
in writing by a majority of the members of the Committee (if consistent with
applicable state law), shall be the valid acts of the Committee.  From time to
time the Board may increase the size of the Committee and appoint additional
members thereof, remove members (with or without cause) and appoint new members
in substitution therefor, fill vacancies however caused, or remove all members
of the Committee and thereafter directly administer the Plan.

       C.   Grant of Stock Rights to Board Members.  Stock Rights may be granted
            --------------------------------------                              
to members of the Board consistent with the provisions of the first sentence of
paragraph 2(A) above, if applicable.  All grants of Stock Rights to members of
the Board shall in all other respects be made in accordance with the provisions
of this Plan applicable to other eligible persons.  Consistent with the
provisions of the first sentence of paragraph 2(A) above, members of the Board
who are either (i) eligible for Stock Rights pursuant to the Plan or (ii) have
been granted Stock Rights may vote on any matters affecting the administration
of the Plan or the grant of any Stock Rights pursuant to the Plan, except that
no such member shall act upon the granting to himself of Stock Rights, but any
such member may be counted in determining the existence of a quorum at any
meeting of the Board during which action is taken with respect to the granting
to him of Stock Rights.

  3.   Eligible Employees and Others.  The maximum number of shares of Common
       -----------------------------                                         
Stock that may be issued to any officer, director, employee or consultant
pursuant to the Plan is 1,400,000 shares.  ISOs may be granted to any employee
of the Company or any Related Corporation.  Those officers and directors of the
Company who are not employees may not be granted ISOs under the Plan.  Non-
Qualified Options, Awards and authorizations to make Purchases may be granted to
any employee, officer or director (whether or not also an employee)

                                      -2-
<PAGE>
 
or consultant of the Company or any Related Corporation. The Committee may take
into consideration a recipient's individual circumstances in determining whether
to grant an ISO, a Non-Qualified Option, an Award or an authorization to make a
Purchase. Granting of any Stock Right to any individual or entity shall neither
entitle that individual or entity to, nor disqualify him from, participation in
any other grant of Stock Rights.

  4.   Stock.  The stock subject to Options, Awards and Purchases shall be
       -----                                                              
authorized but unissued shares of Common Stock of the Company, par value $.01
per share (the "Common Stock"), or shares of Common Stock reacquired by the
Company in any manner.  The aggregate number of shares which may be issued
pursuant to the Plan is 4,585,500 subject to adjustment as provided in paragraph
13; provided, however, that such number of shares shall not be subject to
    -----------------                                                    
adjustment by reason of the four for one stock split in the form of a stock
dividend declared by the Board of Directors of the Company at a meeting on March
27, 1992.  Any such shares may be issued as ISOs, Non-Qualified Options or
Awards, or to persons or entities making Purchases, so long as the number of
shares so issued does not exceed such number, as adjusted.  If any Stock Right
granted under the Plan shall expire or terminate for any reason without having
been exercised in full or shall cease for any reason to be exercisable in whole
or in part, the unissued shares subject to such Stock Options shall again be
available for grants of Stock Rights under the Plan.  For the purposes of the
foregoing sentence, shares withheld from the Stock Right exercise to pay the
exercise price and/or tax consequences of the exercise shall be deemed to have
been issued.

  5.   Granting of Stock Rights.  Stock Rights may be granted under the Plan at
       ------------------------                                                
any time on or after March 27, 1992 and prior to March 27, 2002.  The date of
grant of a Stock Right under the Plan will be the date specified by the
Committee at the time it grants the Stock Right; provided, however, that such
date shall not be prior to the date on which the Committee acts to approve the
grant.  The Committee shall have the right, with the consent of the optionee, to
convert an ISO granted under the Plan to a Non-Qualified Option pursuant to
paragraph 16.

  6.   Minimum Option Price; ISO Limitations.
       ------------------------------------- 

          A. Price for Non-Qualified Options.  The exercise price per share
             -------------------------------                               
   specified in the agreement relating to each Non-Qualified Option granted
   under the Plan shall in no event be less than the minimum legal consideration
   required therefor under the laws of Delaware or the laws of any jurisdiction
   in which the Company or its successors in interest may be organized.

          B. Price for ISOs.  The exercise price per share specified in the
             --------------                                                
   agreement relating to each ISO granted under the Plan shall not be less than
   the fair market value per share of Common Stock on the date of such grant.
   In the case of an ISO to be granted to an employee owning stock possessing
   more than ten percent (10%) of the total combined voting power of all classes
   of stock of the Company or any Related Corporation, the price per share
   specified in the agreement relating to such ISO shall not be less than one
   hundred ten percent (110%) of the fair market value per share of Common Stock
   on the date of grant.

                                      -3-
<PAGE>
 
          C. $100,000 Annual Limitation on ISOs.  Each eligible employee may be
             ----------------------------------                                
   granted ISOs only to the extent that, in the aggregate under this Plan and
   all incentive stock option plans of the Company and any Related Corporation,
   such ISOs do not become exercisable for the first time by such employee
   during any calendar year in a manner which would entitle the employee to
   purchase more than $100,000 in fair market value (determined at the time the
   ISOs were granted) of Common Stock in that year.  Any options granted to an
   employee in excess of such amount will be granted as Non-Qualified Options.

          D.  Determination of Fair Market Value.  If, at the time an Option is
              ----------------------------------                               
   granted under the Plan, the Company's Common Stock is publicly traded, "fair
   market value" shall be determined as of the last business day for which the
   prices or quotes discussed in this sentence are available prior to the date
   such Option is granted and shall mean (i) the average (on that date) of the
   high and low prices of the Common Stock on the principal national securities
   exchange on which the Common Stock is traded, if the Common Stock is then
   traded on a national securities exchange; or (ii) the last reported sale
   price (on that date) of the Common Stock on the NASDAQ National Market List,
   if the Common Stock is not then traded on a national securities exchange; or
   (iii) the closing bid price (or average of bid prices) last quoted (on that
   date) by an established quotation service for over-the-counter securities, if
   the Common Stock is not reported on the NASDAQ National Market List.
   However, if the Common Stock is not publicly traded at the time an Option is
   granted under the Plan, "fair market value" shall be deemed to be the fair
   value of the Common Stock as determined by the Committee after taking into
   consideration all factors which it deems appropriate, including, without
   limitation, recent sale and offer prices of the Common Stock in private
   transactions negotiated at arm's length.

  7.   Option Duration.  Subject to earlier termination as provided in
       ---------------                                                
paragraphs 9 and 10, each Option shall expire on the date specified by the
Committee, but not more than (i) ten years and one day from the date of grant in
the case of Non-Qualified Options, (ii) ten years from the date of grant in the
case of ISOs generally, and (iii) five years from the date of grant in the case
of ISOs granted to an employee owning stock possessing more than ten percent
(10%) of the total combined voting power of all classes of stock of the Company
or any Related Corporation.  Subject to earlier termination as provided in
paragraphs 9 and 10, the term of each ISO shall be the term set forth in the
original instrument granting such ISO, except with respect to any part of such
ISO that is converted into a Non-Qualified Option pursuant to paragraph 16.

  8.   Exercise of Option.  Subject to the provisions of paragraphs 9 through
       ------------------                                                    
12, each Option granted under the Plan shall be exercisable as follows:

          A. Vesting.  The Option shall either be fully exercisable on the date
             -------                                                           
   of grant or shall become exercisable thereafter in such installments as the
   Committee may specify.

          B. Full Vesting of Installments.  Once an installment becomes
             ----------------------------                              
   exercisable it shall remain exercisable until expiration or termination of
   the Option, unless otherwise specified by the Committee.

                                      -4-
<PAGE>
 
          C. Partial Exercise.  Each Option or installment may be exercised at
             ----------------                                                 
   any time or from time to time, in whole or in part, for up to the total
   number of shares with respect to which it is then exercisable.

          D. Acceleration of Vesting.  The Committee shall have the right to
             -----------------------                                        
   accelerate the date of exercise of any installment of any Option; provided
   that the Committee shall not, without the consent of an optionee, accelerate
   the exercise date of any installment of any Option granted to any employee as
   an ISO (and not previously converted into a Non-Qualified Option pursuant to
   paragraph 16) if such acceleration would violate the annual vesting
   limitation contained in Section 422(d) of the Code, as described in paragraph
   6(C).

  9.  Termination of Employment.  If an ISO optionee ceases to be employed by
      -------------------------                                              
the Company and all Related Corporations other than by reason of death or
disability as defined in paragraph 10, no further installments of his ISOs shall
become exercisable, and his ISOs shall terminate after the passage of ninety
(90) days from the date of termination of his employment, but in no event later
than on their specified expiration dates, except to the extent that such ISOs
(or unexercised installments thereof) have been converted into Non-Qualified
Options pursuant to paragraph 16.  Employment shall be considered as continuing
uninterrupted during any bona fide leave of absence (such as those attributable
to illness, military obligations or governmental service) provided that the
period of such leave does not exceed 90 days or, if longer, any period during
which such optionee's right to reemployment is guaranteed by statute.  A bona
fide leave of absence with the written approval of the Committee shall not be
considered an interruption of employment under the Plan, provided that such
written approval contractually obligates the Company or any Related Corporation
to continue the employment of the optionee after the approved period of absence.
ISOs granted under the Plan shall not be affected by any change of employment
within or among the Company and Related Corporations, so long as the optionee
continues to be an employee of the Company or any Related Corporation.  Nothing
in the Plan shall be deemed to give any grantee of any Stock Right the right to
be retained in employment or other service by the Company or any Related
Corporation for any period of time.

  10.  Death; Disability.
       ----------------- 

          A. Death.  If an ISO optionee ceases to be employed by the Company and
             -----                                                              
   all Related Corporations by reason of his death, any ISO of his may be
   exercised, to the extent of the number of shares with respect to which he
   could have exercised it on the date of his death, by his estate, personal
   representative or beneficiary who has acquired the ISO by will or by the laws
   of descent and distribution, at any time prior to the earlier of the
   specified expiration date of the ISO or 180 days from the date of the
   optionee's death.

          B. Disability.  If an ISO optionee ceases to be employed by the
             ----------                                                  
   Company and all Related Corporations by reason of his disability, he shall
   have the right to exercise any ISO held by him on the date of termination of
   employment, to the extent of the number of shares with respect to which he
   could have exercised it on that date, at any time prior to the earlier of the
   specified expiration date of the ISO or 180 days from the date of the
   termination of the optionee's employment.  For the purposes of the Plan, the
   term "disability" shall mean

                                      -5-
<PAGE>
 
   "permanent and total disability" as defined in Section 22(e)(3) of the Code
   or successor statute.

  11. Assignability.  No Option shall be assignable or transferable by the
      -------------                                                       
optionee except by will or by the laws of descent and distribution.  During the
lifetime of the optionee each Option shall be exercisable only by him.

     12. Terms and Conditions of Options.  Options shall be evidenced by
         -------------------------------                                
   instruments (which need not be identical) in such forms as the Committee may
   from time to time approve.  Such instruments shall conform to the terms and
   conditions set forth in paragraphs 6 through 11 hereof and may contain such
   other provisions as the Committee deems advisable which are not inconsistent
   with the Plan, including restrictions applicable to shares of Common Stock
   issuable upon exercise of Options.  In granting any Non-Qualified Option, the
   Committee may specify that such Non-Qualified Option shall be subject to the
   restrictions set forth herein with respect to ISOs, or to such other
   termination and cancellation provisions as the Committee may determine.  The
   Committee may from time to time confer authority and responsibility on one or
   more of its own members and/or one or more officers of the Company to execute
   and deliver such instruments.  The proper officers of the Company are
   authorized and directed to take any and all action necessary or advisable
   from time to time to carry out the terms of such instruments.

     13. Adjustments.  Upon the occurrence of any of the following events, an
         -----------                                                         
   optionee's rights with respect to Options granted to him hereunder shall be
   adjusted as hereinafter provided, unless otherwise specifically provided in
   the written agreement between the optionee and the Company relating to such
   Option:

          A. Stock Dividends and Stock Splits.  If the shares of Common Stock
             --------------------------------                                
   shall be subdivided or combined into a greater or smaller number of shares or
   if the Company shall issue any shares of Common Stock as a stock dividend on
   its outstanding Common Stock, the number of shares of Common Stock
   deliverable upon the exercise of Options shall be appropriately increased or
   decreased proportionately, and appropriate adjustments shall be made in the
   purchase price per share to reflect such subdivision, combination or stock
   dividend.

          B. Consolidations or Mergers.  If the Company is to be consolidated
             -------------------------                                       
   with or acquired by another entity in a merger, sale of all or substantially
   all of the Company's assets or otherwise (an "Acquisition"), the Committee or
   the board of directors of any entity assuming the obligations of the Company
   hereunder (the "Successor Board"), shall, as to outstanding Options, either
   (i) make appropriate provision for the continuation of such Options by
   substituting on an equitable basis for the shares then subject to such
   Options the consideration payable with respect to the outstanding shares of
   Common Stock in connection with the Acquisition; or (ii) upon written notice
   to the optionees, provide that all Options must be exercised, to the extent
   then exercisable, within a specified number of days of the date of such
   notice, at the end of which period the Options shall terminate; or (iii)
   terminate all Options in exchange for a cash payment equal to the excess of
   the fair

                                      -6-
<PAGE>
 
   market value of the shares subject to such Options (to the extent then
   exercisable) over the exercise price thereof.

          C. Recapitalization or Reorganization.  In the event of a
             ----------------------------------                    
   recapitalization or reorganization of the Company (other than a transaction
   described in subparagraph B above) pursuant to which securities of the
   Company or of another corporation are issued with respect to the outstanding
   shares of Common Stock, an optionee upon exercising an Option shall be
   entitled to receive for the purchase price paid upon such exercise the
   securities he would have received if he had exercised his Option prior to
   such recapitalization or reorganization.

          D. Modification of ISOs.  Notwithstanding the foregoing, any
             --------------------                                     
   adjustments made pursuant to subparagraphs A, B or C with respect to ISOs
   shall be made only after the Committee, after consulting with counsel for the
   Company, determines whether such adjustments would constitute a
   "modification" of such ISOs (as that term is defined in Section 424 of the
   Code) or would cause any adverse tax consequences for the holders of such
   ISOs.  If the Committee determines that such adjustments made with respect to
   ISOs would constitute a modification of such ISOs, it may refrain from making
   such adjustments.

          E. Dissolution or Liquidation.  In the event of the proposed
             --------------------------                               
   dissolution or liquidation of the Company, each Option will terminate
   immediately prior to the consummation of such proposed action or at such
   other time and subject to such other conditions as shall be determined by the
   Committee.

          F. Issuances of Securities.  Except as expressly provided herein, no
             -----------------------                                          
   issuance by the Company of shares of stock of any class, or securities
   convertible into shares of stock of any class, shall affect, and no
   adjustment by reason thereof shall be made with respect to, the number or
   price of shares subject to Options.  No adjustments shall be made for
   dividends paid in cash or in property other than securities of the Company.

          G. Fractional Shares.  No fractional shares shall be issued under the
             -----------------                                                 
   Plan and the optionee shall receive from the Company cash in lieu of such
   fractional shares.

          H. Adjustments.  Upon the happening of any of the events described in
             -----------                                                       
   subparagraphs A, B or C above, the class and aggregate number of shares set
   forth in paragraph 4 hereof that are subject to Stock Rights which previously
   have been or subsequently may be granted under the Plan shall also be
   appropriately adjusted to reflect the events described in such subparagraphs.
   The Committee or the Successor Board shall determine the specific adjustments
   to be made under this paragraph 13 and, subject to paragraph 2, its
   determination shall be conclusive.

  If any person or entity owning restricted Common Stock obtained by exercise of
a Stock Right made hereunder receives shares or securities or cash in connection
with a corporate transaction described in subparagraphs A, B or C above as a
result of owning such restricted Common Stock, such shares or securities or cash
shall be subject to all of the conditions and

                                      -7-
<PAGE>
 
restrictions applicable to the restricted Common Stock with respect to which
such shares or securities or cash were issued, unless otherwise determined by
the Committee or the Successor Board.

  14.  Means of Exercising Stock Rights.  A Stock Right (or any part or
       --------------------------------                                
installment thereof) shall be exercised by giving written notice to the Company
at its principal office address.  Such notice shall identify the Stock Right
being exercised and specify the number of shares as to which such Stock Right is
being exercised, accompanied by full payment of the purchase price therefor (a)
in United States dollars in cash or by check,  (b) at the discretion of the
Committee, through delivery or withholding from the Stock Right exercise of
shares of Common Stock having a fair market value equal as of the date of the
exercise to the cash exercise price of the Stock Right, (c) at the discretion of
the Committee, by delivery of the grantee's personal recourse note bearing
interest payable not less than annually at no less than 100% of the lowest
applicable Federal rate, as defined in Section 1274(d) of the Code, (d) at the
discretion of the Committee and consistent with applicable law, through the
delivery of an assignment to the Company of a sufficient amount of the proceeds
from the sale of the Common Stock acquired upon exercise of the Stock Right and
an authorization to the broker or selling agent to pay that amount to the
Company, which sale shall be at the participant's direction at the time of
exercise, or (e) at the discretion of the Committee, by any combination of (a),
(b), (c) and (d) above.  If the Committee exercises its discretion to permit
payment of the exercise price of an ISO by means of the methods set forth in
clauses (b), (c), (d) or (e) of the preceding sentence, such discretion shall be
exercised in writing at the time of the grant of the ISO in question.
Notwithstanding the foregoing, no employee may pay any part of the exercise
price hereof by delivering shares of Common stock to the Company unless such
Common stock has been owned by such employee free of any substantial risk of
forfeiture for at least six months.  The holder of a Stock Right shall not have
the rights of a shareholder with respect to the shares covered by his Stock
Right until the date of issuance of a stock certificate to him for such shares.
Except as expressly provided above in paragraph 13 with respect to changes in
capitalization and stock dividends, no adjustment shall be made for dividends or
similar rights for which the record date is before the date such stock
certificate is issued.

  15.  Term and Amendment of Plan.  This Plan was adopted by the Board of
       --------------------------                                        
Directors and Stockholders of the Company on March 31, 1992.  The Plan shall
expire at the end of the day on March 27, 2002 (except as to Options outstanding
on that date).  The Board may terminate or amend the Plan in any respect at any
time, except that, without the approval of the stockholders obtained within 12
months before or after the Board adopts a resolution authorizing any of the
following actions:  (a) the total number of shares that may be issued under the
Plan may not be increased materially (except by adjustment pursuant to paragraph
13); (b) the benefits accruing to participants under the Plan may not be
materially increased; (c) the requirements as to eligibility for participation
in the Plan may not be materially modified; (d) the provisions of paragraph 3
regarding eligibility for grants of ISOs may not be modified; (e) the provisions
of paragraph 6(B) regarding the exercise price at which shares may be offered
pursuant to ISOs may not be modified (except by adjustment pursuant to paragraph
13); (f) the expiration date of the Plan may not be extended; and (g) the Board
may not take any action which would cause the Plan to fail to comply with Rule
16b-3.  Except as otherwise provided in this paragraph 15, in no

                                      -8-
<PAGE>
 
event may action of the Board or stockholders alter or impair the rights of a
grantee, without his consent, under any Stock Right previously granted to him.

  16.   Conversion of ISOs into Non-Qualified Options; Termination of ISOs.  The
        ------------------------------------------------------------------      
Committee, at the written request of any optionee, may in its discretion take
such actions as may be necessary to convert such optionee's ISOs (or any
installments or portions of installments thereof) that have not been exercised
on the date of conversion into Non-Qualified Options at any time prior to the
expiration of such ISOs, regardless of whether the optionee is an employee of
the Company or a Related Corporation at the time of such conversion.  Such
actions may include, but not be limited to, extending the exercise period or
reducing the exercise price of the appropriate installments of such ISOs.  At
the time of such conversion, the Committee (with the consent of the optionee)
may impose such conditions on the exercise of the resulting Non-Qualified
Options as the Committee in its discretion may determine, provided that such
conditions shall not be inconsistent with this Plan.  Nothing in the Plan shall
be deemed to give any optionee the right to have such optionee's ISOs converted
into Non-Qualified Options, and no such conversion shall occur until and unless
the Committee takes appropriate action.  The Committee, with the consent of the
optionee, may also terminate any portion of any ISO that has not been exercised
at the time of such termination.

  17.  Application Of Funds.  The proceeds received by the Company from the sale
       --------------------                                                     
of shares pursuant to Options granted and Purchases authorized under the Plan
shall be used for general corporate purposes.

  18.  Governmental Regulation.  The Company's obligation to sell and deliver
       -----------------------                                               
shares of the Common Stock under this Plan is subject to the approval of any
governmental authority required in connection with the authorization, issuance
or sale of such shares.

  19.  Withholding of Additional Income Taxes.  Upon the exercise of a Non-
       --------------------------------------                             
Qualified Option, the grant of an Award, the making of a Purchase of Common
Stock for less than its fair market value, the making of a Disqualifying
Disposition (as defined in paragraph 20) or the vesting of restricted Common
Stock acquired on the exercise of a Stock Right hereunder, the Company, in
accordance with Section 3402(a) of the Code, may require the optionee, Award
recipient or purchaser to pay additional withholding taxes in respect of the
amount that is considered compensation includible in such person's gross income.
The Committee in its discretion may condition (i) the exercise of an Option,
(ii) the grant of an Award, (iii) the making of a Purchase of Common Stock for
less than its fair market value, or (iv) the vesting of restricted Common Stock
acquired by exercising a Stock Right, on the grantee's payment of such
additional withholding taxes.  Payment of such additional withholding taxes
shall be in United States dollars in cash or by check and/or at the discretion
of the Committee, through the delivery of previously held shares of common stock
or withholding from the Stock Right exercise of shares of Common Stock having a
fair market value equal as of the date of exercise to the amount of such
withholding taxes.

  20.  Notice to Company of Disqualifying Disposition.  Each employee who
       ----------------------------------------------                    
receives an ISO must agree to notify the Company in writing immediately after
the employee makes a

                                      -9-
<PAGE>
 
Disqualifying Disposition of any Common Stock acquired pursuant to the exercise
of an ISO. A Disqualifying Disposition is any disposition (including any sale)
of such Common Stock before the later of (a) two years after the date the
employee was granted the ISO, or (b) one year after the date the employee
acquired Common Stock by exercising the ISO. If the employee has died before
such stock is sold, these holding period requirements do not apply and no
Disqualifying Disposition can occur thereafter.

  21.  Governing Law; Construction.  The validity and construction of the Plan
       ---------------------------                                            
and the instruments evidencing Stock Rights shall be governed by the laws of the
State of Delaware, or the laws of any jurisdiction in which the Company or its
successors in interest may be organized.  In construing this Plan, the singular
shall include the plural and the masculine gender shall include the feminine and
neuter, unless the context otherwise requires.

                                      -10-

<PAGE>
 
                                                                     EXHIBIT 5.1
                                                                     -----------



                                 March 21, 1997



PerSeptive Biosystems, Inc.
500 Old Connecticut Path
Framingham, MA   01701

  Re:  Registration Statement on Form S-8
       Relating to the 1992 Stock Plan of PerSeptive
       Biosystems, Inc., as amended (hereinafter the "Plan")

Ladies and Gentlemen:

  Reference is made to the above-captioned Registration Statement on Form S-8
(the "Registration Statement") filed by PerSeptive Biosystems, Inc. (the
"Company") on March 21, 1997 with the Securities and Exchange Commission under
the Securities Act of 1933, as amended, relating to an aggregate of 1,000,000
shares of Common Stock, $.01 par value per share, of the Company issuable
pursuant to the Plan (the "Shares").

  We have examined such documents, certificates, records and matters of law that
we have deemed necessary or appropriate for the purpose of this opinion.

  Based on the foregoing, we are of the opinion that the Shares have been duly
authorized and, when issued and sold in accordance with the Plan, will be
validly issued, fully paid and nonassessable.

  We hereby consent to filing of this opinion as Exhibit 5.1 to the Registration
Statement.

                                    Very truly yours,

 

                                    TESTA, HURWITZ & THIBEAULT, LLP

<PAGE>
 
                                                                    EXHIBIT 23.1
                                                                    ------------

                      Consent of Independent Accountants

To the Directors and Stockholders of PerSeptive Biosystems, Inc.:

     We consent to the incorporation by reference in the Registration Statement
of PerSeptive Biosystems, Inc. on Form S-8, related to the Company's 1992 Stock
Plan, of our reports dated November 20, 1996, on our audits of the consolidated
financial statements and financial statement schedule of PerSeptive Biosystems,
Inc. as of September 30, 1996 and 1995.

                                                        COOPERS & LYBRAND L.L.P.

Boston, Massachusetts
March 21, 1997

<PAGE>
 
                                                                    EXHIBIT 23.2
                                                                    ------------


                       Consent of Independent Accountants



We hereby consent to the incorporation by reference in this Registration
Statement on Form S-8 of our report dated December 28, 1994, except for Note 13,
as to which the date is August 11, 1995, appearing on page F-3 of PerSeptive
Biosystem's Annual Report on Form 10-K for the year ended September 30, 1996. We
also consent to the incorporation by reference of our report on the Financial
Statement Schedule, which appears on page S-2 of such Annual Report
on Form 10-K.



PRICE WATERHOUSE LLP


Boston, Massachusetts
March 21, 1997


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