PIONEER WINTHROP REAL ESTATE INVESTMENT FUND
497, 1995-05-01
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                                             May 1, 1995




VIA ELECTRONIC TRANSMISSION

Securities and Exchange Commission
File Desk
Judiciary Plaza
450 Fifth Street, NW
Washington, DC  20549

     Re:      Pioneer Winthrop Real Estate Investment Fund
              (the "Fund")
              File Nos. 33-65822 and 811-7870
              CIK No. 0000908996


Ladies and Gentlemen:

     Pursuant  to Rule  497(j)  under the  Securities  Act of 1933,  this letter
certifies  that:  (1)  the  form  of  Prospectus  and  Statement  of  Additional
Information  that would have been  required to be filed under  paragraph  (c) of
Rule 497 does not differ from that  contained in the most recent  Post-Effective
Amendment No. 4, dated April 25, 1995;  and (2) that the text of the most recent
Post-Effective Amendment No. 4 has been filed electronically.

     If you have any  questions  concerning  the  foregoing  or the  enclosures,
please call me or Elizabeth Watson collect at (617) 742-7825.

                                             Very truly yours,


                                             /s/Eunice R. Simmons
                                             Eunice R. Simmons
                                             EDGAR Compliance Associate


cc:  Ms. Elizabeth Watson
     Ms. Collette B. Rajotte
     Joseph P. Barri, Esq.
     Leonard Pierce, Esq.




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