LOOMIS SAYLES INVESTMENT TRUST
24F-2NT, 1999-12-22
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<PAGE>

                     U.S. SECURITIES AND EXCHANGE COMMISSION
                             Washington, D.C. 20549

                                   FORM 24F-2

                        Annual Notice of Securities Sold
                             Pursuant to Rule 24f-2

1.     Name and address of issuer:

          Loomis Sayles Investment Trust
          One Financial Center
          Boston, MA 02111

2.     Name of each series or class of funds for which this notice is filed (If
       the Form is being filed for all series and classes of securities of the
       issuer, check the box but do not list the series or classes): /x/

3.     Investment Company Act File Number: 811-8282
       Securities Act File Number: 333-22931

4(a).  Last day of fiscal year for which this notice is filed: September 30,
       1999

4(b).  Check box if this Form is being filed late (I.E., more than 90 calendar
       days after the end of the issuer=s fiscal year).

Note: If the Form is being filed late, interest must be paid on the registration
fee due.

4(c).  Check box if this is the last time the issuer will be filing this Form.

5.     Calculation of registration fee:
<TABLE>
         <S>                                                        <C>
         (i) Aggregate sale price of securities sold during
             the fiscal year pursuant to Section 24(f):
                                                                    $195,162,086

        (ii) Aggregate price of securities redeemed or
             repurchased during the fiscal year:                    $ 42,733,427

       (iii) Aggregate price of securities redeemed or
             repurchased during any prior fiscal year ending no
             earlier than October 11, 1995 that were not
             previously used to reduce registration fees payable
             to the Commission:                                               $0

</TABLE>

<PAGE>
<TABLE>

             <S>                                                    <C>
        (iv) Total available redemption credits [add Items
             5(ii) and 5(iii)]:                                     $ 42,733,427

         (v) Net sales -- if Item 5(i) is greater than Item
             5(iv) [subtract Item 5(iv) from Item 5(i)]:
                                                                    $152,428,659

        (vi) Redemption credits available for use in future
             years - if Item 5(i) is less than Item 5(iv)
             [subtract Item 5(iv) from item 5(i)]:                            $0

             (vii) Multiplier for determining registration fee       x   .000264

      (viii) Registration fee due [multiply Item 5(v) by
             Item 5(vii)] (enter "0" if no fee is due):               $40,241.17
</TABLE>

6.   Prepaid shares

     If the response to Item 5(i) was determined by deducting an amount of
securities that were registered under the Securities Act of 1933 pursuant to
rule 24e-2 as in effect before October 11, 1997, then report the amount of
securities (number of shares or other units) deducted here:_____. If there is a
number of shares or other units that were registered pursuant to rule 24e-2
remaining unsold at the end of the fiscal year for which this form is filed that
are available for use by the issuer in future fiscal years, then state that
number here:_____.

7.   Interest Due:---If this form is being filed more than 90 days after the end
     of the issue=s fiscal year (see Instruction D).

8.   Total of the amount of the registration fee due plus any interest due:
     [line 5 (viii) plus line 7)] $40,241.17

9.   Date the registration fee and any interest was sent to the Commission's
     lock box depository: December 22, 1999



                  Method of Delivery:   X     Wire Transfer
                                       ----
                                              Mail or other Means
                                       ----


                                       2

<PAGE>

                                   SIGNATURES

This report has been signed below by the following persons on behalf of the
issuer and in the capacities and on the dates indicated.

By (Signature and Title)*
                                            \s\ Daniel J. Fuss
                                            -----------------------------------
                                            Daniel J. Fuss, President

Date:  December 22, 1999

* Please print the name and title of the signing officer below the signature.


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