<PAGE>
PROSPECTUS SUPPLEMENT Filed pursuant to Rule 424(b)(3)
(To Prospectus Dated Registration No.333-38482
August 22, 2000)
BIOSPHERE MEDICAL, INC.
2,032,255 SHARES OF COMMON STOCK
---------------
The information in this prospectus supplement concerning the Selling
Stockholders supplements the statements set forth under the caption "Selling
Stockholders" in the prospectus. Capitalized items used and not defined herein
shall have the meanings given to them in the prospectus. The information set
forth under the caption "Selling Stockholders" is supplemented as follows:
SELLING STOCKHOLDERS
We issued the shares of common stock that this prospectus covers,
including the shares of common stock underlying the warrants set forth below, in
private placements in February 2000 and July 2000. We have set forth in the
following table, to our knowledge, information about the selling stockholders as
of June 30, 2000, or such later date as the selling stockholders have provided
the information. We have calculated beneficial ownership based on SEC
requirements, and the information we have included regarding beneficial
ownership is not necessarily indicative of beneficial ownership for any other
purpose. Unless we otherwise indicate below, each stockholder named in the table
has sole voting and investment power with respect to all shares he, she or it
beneficially owns, subject to applicable community property laws. We have based
the percentage calculated for each selling stockholder upon the sum of the
"Common Stock" and "Common Stock Issuable Upon Exercise of Warrants" columns.
John Carnuccio is a director and the President and Chief Executive
Officer of BioSphere; Jean-Marie Vogel is the Chairman of BioSphere; Timothy
Barberich and David Southwell, directors of BioSphere, are President and Chief
Executive Officer and Executive Vice President and Chief Financial Officer,
respectively, of Sepracor, the parent corporation of BioSphere. Sepracor is also
a selling stockholder and we are registering for Sepracor the 454,545 shares it
acquired in our private placement in July 2000. We are party to a corporate
services agreement with Sepracor whereby Sepracor provides to us, for a monthly
fee, accounting, human resources and data processing services. Sepracor is also
the guarantor of our line of credit with a bank. In connection with Sepracor's
guarantee, we are party to a security agreement with Sepracor pursuant to which
we have pledged our assets to Sepracor as collateral for its guarantee to the
bank. Except as set forth in this paragraph, none of the selling stockholders
has held any position or had any material relationship with BioSphere in the
past three years.
<PAGE>
We do not know when or in what amounts the selling stockholders may
offer shares for sale. The selling stockholders may decide not to sell all or
any of the shares that this prospectus covers. Because the selling stockholders
may offer all or some of the shares pursuant to this offering, and because there
are currently no agreements, arrangements or understandings with respect to the
sale of any of the shares that the selling stockholders will hold after
completion of the offering, we cannot estimate the number of the shares that the
selling stockholders will hold after completion of the offering. However, for
purposes of this table, we have assumed that, after completion of the offering,
the selling stockholders will hold none of the shares that this prospectus
covers.
<TABLE>
<CAPTION>
Number of Shares of Common Stock Number of Number of Shares of Common Stock
Beneficially Owned Prior to Offering Shares of Beneficially Owned After Offering
--------------------------------------- Common ---------------------------------------
Stock to be
Number Percent Sold Number Percent
--------------------------- ------- ----------- ---------------------------- -------
Common Stock Common Stock
Issuable Issuable
Name of Selling Upon Exercise Upon Exercise
Stockholders Common Stock of Warrants Common Stock of Warrants
--------------- ------------ ------------- ------------ -------------
<S> <C> <C> <C> <C> <C> <C> <C>
ABS Employees'
Venture Fund
Limited
Partnership 0 6,944 * 6,944 0 0 0
ACI Capital /
BSMD, LLC 16,666 4,166 * 20,832 0 0 0
ACI Capital /
BSMDI, LLC 177,777 44,444 2.1 222,221 0 0 0
Marcia L. Aaron 228 0 * 228 0 0 0
Mark C. Alpert 228 0 * 228 0 0 0
Sanjay Arora 556 0 * 556 0 0 0
Gregory H.
Barnhill 228 0 * 228 0 0 0
Timothy J.
Barberich 75,777(1) 6,944 * 34,721 48,000(1) 0 *
Douglas A. Berl
As His Separate
Property #2 228 0 * 228 0 0 0
George A.
Bicher III 228 0 * 228 0 0 0
Biopergs, LLC 27,777 6,944 * 34,721 0 0 0
</TABLE>
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<PAGE>
<TABLE>
<CAPTION>
Number of Shares of Common Stock Number of Number of Shares of Common Stock
Beneficially Owned Prior to Offering Shares of Beneficially Owned After Offering
--------------------------------------- Common ---------------------------------------
Stock to be
Number Percent Sold Number Percent
--------------------------- ------- ----------- ---------------------------- -------
Common Stock Common Stock
Issuable Issuable
Name of Selling Upon Exercise Upon Exercise
Stockholders Common Stock of Warrants Common Stock of Warrants
--------------- ------------ ------------- ------------ -------------
<S> <C> <C> <C> <C> <C> <C> <C>
William A.
Boykin IV &
Ruth Anne Boykin 228 0 * 228 0 0 0
Stephen E. Brady 228 0 * 228 0 0 0
James S.
Brumfield 228 0 * 228 0 0 0
James F.
Buckley, Jr. &
Jane A. Buckley
JT TEN
Community
Property 228 0 * 228 0 0 0
R. William
Burgess, Jr. 2,778 0 * 2,778 0 0 0
CCL Fund LLC 18,182 0 * 18,182 0 0 0
John M.
Carnuccio 89,955(1) 1,388 * 6,943 84,400(1) 0 *
Scott Elaine
Case 228 0 * 228 0 0 0
Castle Creek
Healthcare
Partners LLC 72,728 0 * 72,728 0 0 0
Cerberus
Partners, L.P. 55,556 13,889 * 69,445 0 0 0
Cerberus
International,
LTD. 116,112 27,778 1.4 138,890 5,000 0 *
David F.
Childers, Jr.
VENTURE ACCOUNT 228 0 * 228 0 0 0
Lee Chimerakis
& Theresa
Chimerakis 228 0 * 228 0 0 0
Raymond M.
Clark Jr.
Separate
Property 228 0 * 228 0 0 0
</TABLE>
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<PAGE>
<TABLE>
<CAPTION>
Number of Shares of Common Stock Number of Number of Shares of Common Stock
Beneficially Owned Prior to Offering Shares of Beneficially Owned After Offering
--------------------------------------- Common ---------------------------------------
Stock to be
Number Percent Sold Number Percent
--------------------------- ------- ----------- ---------------------------- -------
Common Stock Common Stock
Issuable Issuable
Name of Selling Upon Exercise Upon Exercise
Stockholders Common Stock of Warrants Common Stock of Warrants
--------------- ------------ ------------- ------------ -------------
<S> <C> <C> <C> <C> <C> <C> <C>
Brett D.
Clifford 228 0 * 228 0 0 0
J. Michael
Connelly 228 0 * 228 0 0 0
Fred C. C.
Crozier 228 0 * 228 0 0 0
Scott A. Davey
Venture Acct. 228 0 * 228 0 0 0
John W. Davis
III 228 0 * 228 0 0 0
Robert J.
Deshong &
Patricia A.
Deshong
JT TEN 228 0 * 228 0 0 0
Jeffrey A.
DeYoung 228 0 * 228 0 0 0
Domain Public
Equity Partners
L.P. 80,000 0 * 80,000 0 0 0
Thomas U.
Dudley II 228 0 * 228 0 0 0
Keith R. Dull &
Susan G. Dull,
JT TEN 228 0 * 228 0 0 0
Robert B. East 228 0 * 228 0 0 0
Jonathan E.
Farber & Bonnie
S. Farber
JT WROS 228 0 * 228 0 0 0
George R. Floyd 228 0 * 228 0 0 0
Ivy T. Fradin 228 0 * 228 0 0 0
Donald E.
Froude SPECIAL
ACCOUNT 228 0 * 228 0 0 0
</TABLE>
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<PAGE>
<TABLE>
<CAPTION>
Number of Shares of Common Stock Number of Number of Shares of Common Stock
Beneficially Owned Prior to Offering Shares of Beneficially Owned After Offering
--------------------------------------- Common ---------------------------------------
Stock to be
Number Percent Sold Number Percent
--------------------------- ------- ----------- ---------------------------- -------
Common Stock Common Stock
Issuable Issuable
Name of Selling Upon Exercise Upon Exercise
Stockholders Common Stock of Warrants Common Stock of Warrants
--------------- ------------ ------------- ------------ -------------
<S> <C> <C> <C> <C> <C> <C> <C>
Barry L. Garber
& Michele Lax
JT TEN 228 0 * 228 0 0 0
David G.
Ghysels, Jr. 228 0 * 228 0 0 0
Stephen R.
Goldstein 228 0 * 228 0 0 0
David M. Gray 228 0 * 228 0 0 0
Douglas C. W.
Greenwood 228 0 * 228 0 0 0
Benjamin H.
Griswold IV 228 0 * 228 0 0 0
Burton S.
Halpert 228 0 * 228 0 0 0
John K. Hollan 228 0 * 228 0 0 0
F. Mackey Hughes 228 0 * 228 0 0 0
Michael W. Hyde
& Barbara A.
Hyde JT TEN 228 0 * 228 0 0 0
John P. Jaeger
& Lynn Miller
Jaeger
Ten Com 228 0 * 228 0 0 0
Richard Judd 228 0 * 228 0 0 0
J. Robert
Judkins 228 0 * 228 0 0 0
Stephen J.
Kelly & Laura
S. Kelly
JT TEN 228 0 * 228 0 0 0
E. Robert
Kent, Jr. 228 0 * 228 0 0 0
Robert S.
Killebrew, Jr. 228 0 * 228 0 0 0
</TABLE>
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<PAGE>
<TABLE>
<CAPTION>
Number of Shares of Common Stock Number of Number of Shares of Common Stock
Beneficially Owned Prior to Offering Shares of Beneficially Owned After Offering
--------------------------------------- Common ---------------------------------------
Stock to be
Number Percent Sold Number Percent
--------------------------- ------- ----------- ---------------------------- -------
Common Stock Common Stock
Issuable Issuable
Name of Selling Upon Exercise Upon Exercise
Stockholders Common Stock of Warrants Common Stock of Warrants
--------------- ------------ ------------- ------------ -------------
<S> <C> <C> <C> <C> <C> <C> <C>
Mark Klausner &
Melinda Klausner 556 0 * 556 0 0 0
Lynn I. Larson 228 0 * 228 0 0 0
Douglas
Bermingham and
George Wilson,
Trustees for
the benefit of
Jane Macrae 10,000 0 * 10,000 0 0 0
Matthew W.
Madlinger &
Kimberly A.
Madlinger
JT TEN 228 0 * 228 0 0 0
Andrew W. Marcus 228 0 * 228 0 0 0
Brian T.
Marshall 228 0 * 228 0 0 0
Mark J. McHugh 227 0 * 227 0 0 0
Daniel E.
McIntyre &
Cynthia A.
McIntyre
TEN ENT 227 0 * 227 0 0 0
Jeffrey Meyer 228 0 * 228 0 0 0
L. Gartrell
Milam III TTEE
FBO
L. Gartrell
Milam III
REVOCABLE TRUST
DTD 9/14/92 228 0 * 228 0 0 0
Brent Milner &
Michele Milner 2,778 0 * 2,778 0 0 0
Stephen T.
Mitchell &
Cecelia P.
Mitchell
JT TEN 228 0 * 228 0 0 0
</TABLE>
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<PAGE>
<TABLE>
<CAPTION>
Number of Shares of Common Stock Number of Number of Shares of Common Stock
Beneficially Owned Prior to Offering Shares of Beneficially Owned After Offering
--------------------------------------- Common ---------------------------------------
Stock to be
Number Percent Sold Number Percent
--------------------------- ------- ----------- ---------------------------- -------
Common Stock Common Stock
Issuable Issuable
Name of Selling Upon Exercise Upon Exercise
Stockholders Common Stock of Warrants Common Stock of Warrants
--------------- ------------ ------------- ------------ -------------
<S> <C> <C> <C> <C> <C> <C> <C>
W. Christopher
Mortenson, Jr. 228 0 * 228 0 0 0
Michael Murray
& Sarah M.
Murray 228 0 * 228 0 0 0
Patrick A.
O'Shea 228 0 * 228 0 0 0
Scott Olsen 228 0 * 228 0 0 0
Michael T. Ott 228 0 * 228 0 0 0
J. Ralph Parker 227 0 * 227 0 0 0
David K. Parse 228 0 * 228 0 0 0
James S.
Patricelli &
Sharon
Patricelli 228 0 * 228 0 0 0
Pequod
Investments,
L.P. 200,000 25,000 2.1 149,800 50,200 0 *
Pequod
International,
LTD 125,000 16,667 1.3 103,435 38,232 0 *
Pharma w/Health 136,363 0 1.3 136,363 0 0 0
Eric T. Philippi 227 0 * 227 0 0 0
Roger G. Powell 227 0 * 227 0 0 0
John R. Radle 227 0 * 227 0 0 0
Richard Gallen
& Co. Pension
Trust 002 11,112 2,778 * 13,890 0 0 0
Richter
Investment Corp. 5,000 0 * 5,000 0 0 0
Philip John
Rauch 228 0 * 228 0 0 0
</TABLE>
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<PAGE>
<TABLE>
<CAPTION>
Number of Shares of Common Stock Number of Number of Shares of Common Stock
Beneficially Owned Prior to Offering Shares of Beneficially Owned After Offering
--------------------------------------- Common ---------------------------------------
Stock to be
Number Percent Sold Number Percent
--------------------------- ------- ----------- ---------------------------- -------
Common Stock Common Stock
Issuable Issuable
Name of Selling Upon Exercise Upon Exercise
Stockholders Common Stock of Warrants Common Stock of Warrants
--------------- ------------ ------------- ------------ -------------
<S> <C> <C> <C> <C> <C> <C> <C>
H. Thompson
Rodman, Jr. &
Mariella
Smith-Masters
JT TEN 227 0 * 227 0 0 0
Richard M. Ross 228 0 * 228 0 0 0
William R.
Rothe & Gay
Frederick Rothe
TEN ENT SPECIAL
ACCOUNT 227 0 * 227 0 0 0
Rudman
Partners, L.P. 90,909 0 * 90,909 0 0 0
James S. Sale 227 0 * 227 0 0 0
Bryant W.
Seaman III &
Rebecca M.
Seaman 228 0 * 228 0 0 0
Sepracor Inc. 5,824,333 0 55.6 454,545 5,369,788 0 51.3
David P.
Southwell 13,555(1) 1,388 * 6,943 8,000(1) 0 *
John A.
Spilman IV 228 0 * 228 0 0 0
David Steinberg 2,778 0 * 2,778 0 0 0
Kathryn K.
Stewart 556 0 * 556 0 0 0
Summer Hill
Partners L.P. 45,455 0 * 45,455 0 0 0
Thomas W.
Sutton & Carol
J. Sutton
TEN ENT 228 0 * 228 0 0 0
Teton Partners 50,000 0 * 50,000 0 0 0
Bruce H.
Treitman &
Keyla Treitman 228 0 * 228 0 0 0
</TABLE>
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<PAGE>
<TABLE>
<CAPTION>
Number of Shares of Common Stock Number of Number of Shares of Common Stock
Beneficially Owned Prior to Offering Shares of Beneficially Owned After Offering
--------------------------------------- Common ---------------------------------------
Stock to be
Number Percent Sold Number Percent
--------------------------- ------- ----------- ---------------------------- -------
Common Stock Common Stock
Issuable Issuable
Name of Selling Upon Exercise Upon Exercise
Stockholders Common Stock of Warrants Common Stock of Warrants
--------------- ------------ ------------- ------------ -------------
<S> <C> <C> <C> <C> <C> <C> <C>
United Capital
Management, Inc. 181,818 0 1.7 181,818 0 0 0
Ursus Capital,
L.P. 15,000 3,750 * 18,750 0 0 0
Jean-Marie Vogel 647,215(1) 1,388 5.8 6,943 641,660(1) 0 5.8
Daniel P. Walsh
& Cynthia P.
Walsh 228 0 * 228 0 0 0
Scott A. Wieler 228 0 * 228 0 0 0
Karl A. Will 228 0 * 228 0 0 0
Thomas W.
Winstead, Jr. &
Carol J.
Winstead
TEN ENT 228 0 * 228 0 0 0
James W.
Wohlgemuth &
Caroline Wing
Wohlgemuth
JT TEN 228 0 * 228 0 0 0
David Woolford
& Enjeen L.
Woolford 228 0 * 228 0 0 0
-------------------------
</TABLE>
*Represents beneficial ownership of less than one percent of our common stock.
(1) Includes shares of common stock underlying options which the following
persons may exercise within 60 days of June 30, 2000 in the following amounts:
Timothy J. Barberich, 48,000 shares; John Carnuccio, 74,400 shares; David
Southwell, 7,000 shares; and Jean-Marie Vogel, 607,433 shares. Excludes the
5,824,333 shares of common stock beneficially owned by Sepracor, the parent
corporation of BioSphere, as to which shares Messrs. Barberich, Carnuccio,
Southwell and Vogel disclaim beneficial ownership.
The date of this Prospectus is October 30, 2000.
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