HANCOCK JOHN INSTITUTIONAL SERIES TRUST
24F-2NT, 1995-04-27
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<PAGE>   1

                                 [LETTERHEAD]


                                                                  April 19, 1995


United States Securities and Exchange Commission
OFICS Filer Support
SEC Operations Center
6432 General Green Way
Alexandria, Virginia. 22312-2413

Re:     Rule 24f-2 Notice for
        John Hancock Institutional Series Trust
           -  John Hancock Berkeley Bond Fund
           -  John Hancock Berkeley Dividend Performers Fund
           -  John Hancock Berkeley Fundamental Value Fund
           -  John Hancock Berkeley Global Bond Fund
           -  John Hancock Berkeley Overseas Growth Fund
           -  John Hancock Berkeley Sector Opportunity Fund
           -  John Hancock Independence Balanced Fund
           -  John Hancock Independence Diversified Core Equity Fund II
           -  John Hancock Independence Growth Fund
           -  John Hancock Independence Medium Capitalization Fund
           -  John Hancock Independence Value Fund
        Registration No. 33-86102     811-8852
        Account No. 0000932683
        CIK: 932683

Dear Gentlemen:

The purpose of this letter is to notify the Commission within two (2) months
of the end of the Registrant's Fiscal Year of the number of Registrant's shares
sold during the last fiscal year which are to be registered pursuant to Rule
24f-2 and to pay the appropriate registration fee.

The information required by the above referenced rule is as follows:

Fiscal Year:                               Inception to February 28, 1995

        Number of Shares previously
        Registered Other than
        Pursuant to Rule 24f-2
        Remaining Unsold at
        Beginning of Fiscal Year:                                       0

        Number of Shares registered
        During Fiscal Year other than
        Pursuant to Rule 24f-2:                                         0

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United States Securities and Exchange Commission
John Hancock Institutional Series Trust
April 19, 1995
Page Two

        Number of Shares Sold During
        Fiscal Year:                                                    0


        Number of Shares Sold During
        Fiscal Year Pursuant to
        Rule 24f-2:                                                     0




Attached to this Rule 24f-2 Notice, and made part hereof, is an opinion of
counsel indicating that the Securities, the Registration of which the
notice makes definite in number, were legally issued, fully paid and
non-assessable by the Registrant.

In accordance with subsection (c) of Rule 24f-2, no registration fee for John
Hancock Institutional Series Trust is due to the Securities and Exchange
Commission. The fee computation is based upon the following:



    (1) Aggregate Sales Price of Shares
        Sold During the
        Fiscal Year in Reliance Upon
        the 24f-2 Declaration (Based
        on aggregate sale price for
        all shares sold of $0 less
        aggregate sale price of shares
        registered pursuant to Rule 24e-2
        of $None)                                                       $0

Reduced By

    (1) Aggregate Redemption Price
        of Shares Redeemed During
        the Fiscal Year and,                                            (0)

    (2) Aggregate Redemption
        Price of Redeemed Shares
        Previously Applied by
        Fund Pursuant to Rule
        24e-2 (a) Filings Made Pursuant
        to Section 24(e)(1) of Investment
        Company Act of 1940                                             $0
                                                             -------------


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United States Securities and Exchange Commission
John Hancock Institutional Series Trust
April 19, 1995
Page Three

        Net sales applicable for fee                                    $0
        Computation of fee                                      X.00034483
                                                             -------------
        Fee                                                          $0.00
                                                             -------------










Any questions regarding this matter should be addressed to James J. Stokowski,
Treasurer, John Hancock Advisers, Inc., 101 Huntington Avenue, 8th floor,
Boston, MA  02199-7603, (617) 375-1652.


                                                Very truly yours,

                                                /s/ Joan E. Arnold

                                                Joan E. Arnold
                                                Administrative Officer
                                                Mutual Funds


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