HANCOCK JOHN INSTITUTIONAL SERIES TRUST
24F-2NT, 1996-04-24
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                    U.S. SECURITIES AND EXCHANGE COMMISSION
                             Washington, D.C. 20549

                                   FORM 24F-2
                        Annual Notice of Securities Sold
                             Pursuant to Rule 24f-2

             Read instructions at end of Form before preparing Form.
                             Please print or type

- --------------------------------------------------------------------------------

1.   Name and address of issuer:
                     John Hancock Institutional Series Trust
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2.   Name of each series or class of funds for which this notice is filed:
          Independence  Balanced Fund,  Independence  Growth Fund,  Independence
          Medium  Capitalization  Fund,  Independence  Value Fund,  Independence
          Diversified Core Equity Fund II, Dividend Performers Fund, Active Bond
          Fund, Global Bond Fund,  Multi-Sector  Growth Fund,  Fundamental Value
          Fund, International Equity Fund, Small Capitalization Equity Fund
- --------------------------------------------------------------------------------
3.   Investment Company Act File Number:
                     811-8852
    Securities Act File Number:
                     33-86102
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4.   Last day of fiscal year for which this notice is filed
                     February 29, 1996
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5.   Check box if this  notice is being filed more than 180 days after the close
     of the issuer's fiscal year for purposes of reporting securities sold after
     the close of the fiscal year but before  termination  of the issuer's 24f-2
     declaration:
                                                  [    ]
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6.   Date of termination  of issuer's  declaration  under rule 24f-2 (a)(1),  if
     applicable (see Instruction A.6):    
                 N/A
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7.   Number and amount of  securities of the same class or series which had been
     registered  under the  Securities  Act of 1933 other than  pursuant to rule
     24f-2 in a prior fiscal year, but which remained unsold at the beginning of
     the fiscal year:
                                0 shares
                     $          0
- --------------------------------------------------------------------------------
8.   Number and amount of  securities  registered  during the fiscal  year other
     than pursuant to rule 24f-2:
                                0 shares
                     $          0
- --------------------------------------------------------------------------------
9.   Number and aggregate sales price of securities sold during the fiscal year:
                       22,287,024 shares
                     $210,688,189
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<PAGE>

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10.  Number and aggregate  sales price of securities sold during the fiscal year
     in reliance upon registration pursuant to rule 24f-2:
                        22,287,024 shares
                      $210,688,189
- --------------------------------------------------------------------------------
11.  Number and  aggregate  sales price of  securities  issued during the fiscal
     year in connection  with dividend  reinvestment  plans,  if applicable (see
     Instruction B.7):
                           184,582 shares
                      $  1,850,766
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12.  Calculation of registration fee:

     (i)  Aggregate  sale price of  securities sold  
          during the fiscal year in reliance on rule 
          24f-2 (from Item 10):

                                                                    $210,688,189
                                                                     -----------

     (ii) Aggregate price of shares issued in connection 
          with dividend reinvestment plans (from Item 11, 
          if applicable):                                           +  1,850,766
                                                                     -----------

     (iii)Aggregate price of shares redeemed or repurchased 
          during the fiscal year (if applicable):                   - 19,128,572
                                                                     -----------

     (iv) Aggregate price of shares redeemed or repurchased 
          and previously applied as a reduction to filing 
          fees pursuant to rule 24e-2 (if applicable):              +          0
                                                                     -----------

     (v)  Net aggregate price of securities sold and issued 
          during the fiscal year in reliance on rule 24f-2 
          [line (i), plus line (ii), less line (iii), plus 
          line (iv)] (if applicable):                                193,410,383
                                                                     -----------

     (vi) Multiplier prescribed by Section 6(b) of the 
          Securities Act of 1933 or other applicable law or
          regulation (see Instruction C.6):                         x     1/2900
                                                                     -----------

     (vii)Fee due [line (i) or line (v) multiplied by line (vi)]:   $     66,693
                                                                     ===========

Instruction: Issuers should complete lines (ii), (iii), (iv) and (v) only if the
             form is being filed within 60 days after the close of the issuer's
             fiscal year.                         See Instruction C.3
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13.  Check box if fees are being remitted to the Commission's lockbox depository
     as described in section 3a of the Commission's  Rules of Informal and Other
     Procedures (17 CFR 202.3a).
                                                  [  X ]

     Date of mailing or wire transfer of filing fees to the Commission's lockbox
     depository:
                          April 23, 1996
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<PAGE>

- --------------------------------------------------------------------------------
                                   SIGNATURES

     This report has been signed below by the following persons on behalf of the
     issuer and in the capacities and on the dates indicated:

                                           /s/ Paul L. Aries
      By (Signature and Title) *           -------------------------------------

                                           Paul L. Aries     Assistant Treasurer

      Date:   April 19,1996
              ------------------------------------------

  * Please print the name and title of the signing officer below the signature
- --------------------------------------------------------------------------------
<PAGE>

[PRINTED ON JOHN HANCOCK LETTERHEAD]




April 17, 1996


John Hancock Institutional Series Trust
101 Huntington Avenue
Boston, MA  02199


Re:      Rule 24f-2 Notice for John Hancock Institutional Series Trust
         (File Nos.  33-86102;  811-8852) (0000932683)

Ladies and Gentlemen:

In  connection  with the filing of a Notice  pursuant  to Rule  24f-2  under the
Investment  Company Act of 1940, as amended,  making  definite the  registration
under  the  Securities  Act  of  1933  of  22,471,606  shares  of  John  Hancock
Institutional  Series Trust (the  "Trust") sold in reliance upon said Rule 24f-2
during  the  fiscal  year  ended  February  29,  1996 it is the  opinion  of the
undersigned that such shares were legally issued, fully paid and nonassessable.

In  connection  with this opinion it should be noted that the Trust is an entity
of  the  type  generally  known  as  a  "Massachusetts  business  trust."  Under
Massachusetts  law,  shareholders of a Massachusetts  business trust may be held
personally  liable  for the  obligations  of the  Trust.  However,  the  Trust's
Declaration  of Trust  disclaims  shareholder  liability for  obligations of the
Trust and indemnifies any shareholder of the Trust, with such indemnification to
be paid solely out of the assets of the Trust. Therefore, the shareholder's risk
is limited to circumstances in which the assets of the Trust are insufficient to
meet the obligations asserted against such assets.


Sincerely,

JOHN HANCOCK ADVISERS, INC.

/s/ Theresa Apruzzese

Theresa Apruzzese
Assistant Secretary
Member of Massachusetts Bar




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